固定化蛋白酶在羊毛织物防毡缩整理中的应用  

Applied immobilizated protease to wool fabric for anti-felting

作　　者：张弛[1,2] 曹秀[1,2] 杜壮[1,2] 阎克路[1,2] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620 [2]国家染整工程技术研究中心,上海201620

出　　处：《毛纺科技》2014年第12期1-6,共6页Wool Textile Journal

摘　　要：以甲基丙烯酸和甲基丙烯酸甲酯共聚物（Eudragit L100）作为载体,实现蛋白酶的固定化并用于羊毛的防毡缩整理.研究采用红外光谱证明了聚合物Eudragit L100的羧基与蛋白酶氨基通过酰胺键键合实现蛋白酶的固定化;SDS-PAGE凝胶电泳结果表明蛋白酶的分子质量从4.1～6.5 kDa增大至固定化后45 kDa以上;固定化蛋白酶的比活性为13.69 U/mg.同时,分析了固定化蛋白酶整理前后羊毛织物的差热扫描量热（DSC）曲线,电子扫描电镜（SEM）照片和织物的抗毡缩性能和力学性能等结果.结果表明,固定化蛋白酶整理羊毛织物减少了羊毛纤维的α-结晶度;固定化蛋白酶对纤维的水解作用控制在纤维表面,减少了对纤维的损伤;织物的面积毡缩率从原毛织物的21.52％降至0.89％;失重率较蛋白酶整理羊毛织物降低21.90％;拉伸断裂强力较蛋白酶整理羊毛织提高13.10％,证明固定化蛋白酶整理羊毛织物不仅赋予织物良好的防毡缩性能,而且保持了良好的服用性能.Applied polymer Eudragit L100 as carrier to immobilizate protease for wool antifelting. Immobilizated protease was analyzed by FTIR and SDS-PAGE. The results showed that immobilizted protease was form via covalent bonding and molecular weight of protease was increased from 4.1 6.5 kDa to over 45 kDa. The crystal structure of wool treated with immobilizted protease were measured by means of differential scanning calorimetry （DSC）. The results revealed that the α-crystallinity was decreased. Meanwhile, we employed scanning electron microscope （SEM） to observe the morphology of treated wool fiber. Compared with the wool fabric treated with protease, the wool fabric treated with immobilizated protease offered remarkable benefits： weight reduction rate reduced by 21.90% , tensile strength increased by 13. 10%, and area shrinkage reduced from the original 25.21% to 0. 89%, indicating that wool fiber damage is significantly reduced and excellent antifelting property imparted to the wool.

关 键 词：蛋白酶 固定化 羊毛织物 防毡缩 

分 类 号：TS195.56[轻工技术与工程—纺织化学与染整工程]