紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量  

Determination of total triterpenes from the roots of Sonchus arvensis L.by spectrophotometry

作　　者：刘伟锐[1] 张霞[1] 姜蕊[1] 杨悦[1] 谢梦[1] 颜承[1] 折改梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《西北药学杂志》2014年第6期554-557,共4页Northwest Pharmaceutical Journal

基　　金：教育部大学生创新性实验计划(编号:201310026068);陕北地区苦菜汁开发研究(横向课题)

摘　　要：目的：用紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量。方法采用AB-8、D-101、D-4020、HPD-400和NKA-95种大孔树脂对苣荬菜根部总三萜皂苷进行纯化，以齐墩果酸为对照品，50 g·L^-1香草醛-冰醋酸、高氯酸为显色系统，在546 nm波长处测定总三萜皂苷含量。结果在AB-8、D-101、D-4020、HPD-400和NKA-95种树脂中，D-101对苣荬菜根部总三萜皂苷纯化效果最好；齐墩果酸质量浓度在0．0342～0．1065mg·mL^-1范围内，吸光度与含量呈良好的线性关系，回归方程Y ＝8．2982 X-0．0849（r＝0．9996），平均加样回收率为95．3％，RSD＝1．5％（n＝5），苣荬菜根部总三萜皂苷含量为0．202％，RSD＝2．6％。结论该方法操作简单，快速灵敏，准确可靠，对设备要求不高，适用于苣荬菜根部总三萜皂苷的含量测定。Objective To establish a new method for the determination of total triterpenes from the roots of Sonchus arvensis L .by spectrophotometry .Methods By using AB-8 ,D-101 ,D-4020 ,HPD-400 and NKA-9 five kinds of macroporous resins for the pur-ification of total triterpenes from the roots of S .arvensis L .,with oleanolic acid as standard ,50 g · L -1 vanillin-acetic acid ,per-chloric acid as coloring systems ,triterpenes was measured by spectrophotometry and detected at 546 nm .Results D-101 macro-porous resin showed the best performance for the purification of the total triterpenes from the roots of S .arvensis L ..Absorbance and content of oleanolic acid showed a good linear relationship in the range of 0 .034 2-0 .106 5 mg · mL-1 .The regression equation was Y=8 .298 2X-0 .084 9（r=0 .999 6） ,and the average recovery rate was 95 .3% ,RSD=1 .5% （n=5） .The content of total triter-penes in the roots of S .arvensis L .was 0 .202% ,RSD = 2 .6% .Conclusion This method is simple ,fast and sensitive ,accurate and re-liable ,less demanding on the device ,and can be used for the content determination of total triterpenes from the roots of S .arvensis L ..

关 键 词：苣荬菜 总三萜皂苷 含量测定 紫外可见分光光度法 

分 类 号：R282[医药卫生—中药学]