HPLC-ELSD法测定含三七中成药的皂苷成分的含量  

Determination of saponins in Chinese patent drugs by HPLC-ELSD

作　　者：王芮[1] 郭华[2] 

机构地区：[1]解放军307医院药学部,北京100071 [2]军事医学科学院门诊部,北京100850

出　　处：《西北药学杂志》2014年第6期575-577,共3页Northwest Pharmaceutical Journal

摘　　要：目的：建立三七成分中皂苷成分的测定方法。方法采用 HPLC-ELSD 法测定三七皂苷 R1、人参皂苷 Rg1和 Rb1的含量。结果3个成分分离效果很好，线性关系良好。回收率测定结果在95．6％～105．0％之间。结论所建立的方法简便、准确、重复性好，可用于含三七的中成药中皂苷成分的质量控制。Objective To establish a method for the determination of saponins in Chinese patent drugs .Methods Notoginsenoside R1 ,ginsenosides Rg1 and Rb1 were determined by using HPLC-ELSD .Results Notoginsenoside R1 ,ginsenosides Rg1 and Rb1 were well separated .The recoveries were between 95 .6% and 105 .0% .Conclusion The method is simple ,accurate with good re-peatability and can be used for the quality control for Chinese patent drugs .

关 键 词：三七皂苷R1 人参皂苷RG1 人参皂苷RB1 HPLC-ELSD 

分 类 号：R917[医药卫生—药物分析学]