耐碳青霉烯类肺炎克雷伯菌的耐药机制研究  

The mechanisms of resistance of carbapenem-resistant Klebsiella pneumoniae

作　　者：李桂秋[1] 崔兰英[1] 陈淑兰[1] 郭大文[1] 赵金英[1] 路娟[1] 

机构地区：[1]哈尔滨医科大学第一附属医院检验科,黑龙江哈尔滨150001

出　　处：《中国微生态学杂志》2014年第11期1272-1274,共3页Chinese Journal of Microecology

基　　金：黑龙江省青年基金(QC2010071)

摘　　要：目的了解碳青霉烯类耐药肺炎克雷伯菌及耐药机制。方法对2012-2013年临床分离的耐碳青霉烯类肺炎克雷伯菌共计12株进行分析,药敏采用MIC方法检测,用WHONET 5.6软件进行分析,KPC表型检测采用改良Hodge试验,基因检测采用PCR方法。结果 12株碳青霉烯类耐药肺炎克雷伯菌改良Hodge试验阴性,基因测序为KPC-2型。结论 KPC-2基因是引起本院肺炎克雷伯菌耐药的主要原因。Objective To understand the mechanisms of carbapenems resistance and drug resistance of Klebsiella pneumoniae. Methods A total of 12 clinical strains of carbapenems-resistant Klebsiella pneumoniae from 2012 to2013 were analyzed,and the MIC method was used for the drug susceptibility test. WHONET 5. 6 software was used for the analysis. KPC phenotypic detection was performed by using modified Hodge test,and genetic test by using PCR. Results The 12 carbapenems-resistant strains was negative to modified Hodge test. Gene sequencing showed that their KPC type were type 2. Conclusion KPC-2 is the main reason causing the drug resistance of Klebsiella pneumoniae in the hospital.

关 键 词：肺炎克雷伯菌 耐药 碳青霉烯酶 

分 类 号：R378.996[医药卫生—病原生物学]