解郁胶囊中白芍提取纯化工艺研究  

Study on the extraction and purification procedure of white peony in Xieyu capsule

作　　者：江斌[1] 何伟[1] 李勇[1] 吉国辉[1] 林伟鑫[1] 

机构地区：[1]广东药学院药物研究所/广东省药物新剂型重点实验室,广东广州510006

出　　处：《广东药学院学报》2014年第5期559-563,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科技计划项目(2011A030100014)

摘　　要：目的优选解郁胶囊中白芍的最佳提取、纯化工艺条件。方法按《中国药典》2010年版规定项下方法测定芍药苷含量,以乙醇体积分数、乙醇用量、提取时间为考察因素,以芍药苷提取率和浸膏得率的综合评分为指标,采用正交试验法优选白芍提取工艺;以比吸附量和洗脱率为指标,采用大孔吸附树脂法对其进行纯化。结果优选提取工艺条件为:体积分数50%乙醇,8倍量,提取2次,每次2.0 h;纯化工艺条件为:选用D101型大孔吸附树脂,调节上样溶液芍药苷质量浓度为19.94 mg·m L-1,最大上样体积为1.5 BV(BV为树脂体积),上样流速为1.0 m L·min-1,以1 BV水洗去杂,体积分数50%乙醇3 BV洗脱,洗脱流速为2.0 m L·min-1。结论优选的提取、纯化工艺条件有效成分提取率高,工艺合理可行。Objective To optimize the extraction and purification procedure of white peony for Xieyu capsule.Methods The content of peony glucoside was determined by HPLC according to Chinese Pharmacopoeia 2010.The extraction technology of white peony was optimized by orthogonal design with the concentration and amount of ethanol and extraction time as factors,and with taking extract yield and the contents of peony glucoside as index.The macroporous adsorption resin was used to purify white peony extract with taking adsorption amount and elution rate as index.Results The optimal extraction procedure was as follows：white peony was extracted two times with 50% alcohol whose volume was 8 times as much as the volume of the extracted medicinal material,and was refluxed for 2.0 h each time.Purification effect of D101 type macroporous was the best,and its optimum technology conditions were：the concentration of peony glucoside sample solution was 19.94 mg · mL^-1,and the maximum sample volume was 1.5 BV（BV was volume of resin）,and the feeding rate was 1.0 mL · min^-1,and it was eluted by 3 BV 50% alcohol at the eluting velocity of 2.0 mL · min^-1.Conclusion The extraction rate of the active components is high by the optimized extraction and purification procedure,and the procedure is reasonable and feasible.

关 键 词：白芍 芍药苷 提取工艺 纯化工艺 

分 类 号：TQ461[化学工程—制药化工]