延龄草中总皂苷的含量测定  

Content determination of total saponins in Trillium tschonoskii Maxim

作　　者：罗方利[1] 郭力[1] 许莉[1] 贝圆[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2014年第2期24-25,共2页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的：建立延龄草总皂苷的含量测定方法。方法：以薯蓣皂苷元为对照品，采用分光光度法，高氯酸显色，在408nm处测定吸光度，计算延龄草中总皂苷的含量。结果：薯蓣皂苷元在0．01～0．06mg·mL^-1的浓度范围内与吸光度值呈良好线性关系（R^2=0．9996），方法的平均回收率为99．43％，RSD为2．64％。结论：建立了延龄草中总皂苷含量测定方法，该方法操作简便、准确、灵敏、重现性好，可用于延龄草的质量控制。Objective： To establish the assay method of total saponins in Trillium tschonoskii Maxim. Method： The total saponins in Trillium tschonoskii Maxim. was calculated with diosgenin as reference. The medicine was in coloration with perchloric acid, and absorbability was detected at 408 nm by adopting spectrophotometric method. Result： The calibration curve was linear in the range of 0.01 mg.mL^-1～0.06 mg.mL1 （R^2 = 0.9996） . The average recovery of diosgenin was 99.43% and RSD was 2.64%. Conclusion： The method is simple, accurate, sensitive and reproducible. It can be applied in the quality control of Trillium tschonoskii Maxim..

关 键 词：延龄草 总皂苷 薯蓣皂苷元 分光光度法 

分 类 号：R282.6[医药卫生—中药学]