HPLC同时测定抗骨增止疼片中淫羊藿苷和芒柄花素含量  

Simultaneous determination of icariin and formonone in kangguzeng Zhiteng tablets

作　　者：高华娟[1] 李希[2] 黄嫣[2] 冯建安[2] 杨华[1] 李远辉[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]四川省中医药科学院中医研究所,四川成都610031

出　　处：《中药与临床》2014年第2期45-47,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:建立HPLC同时测定抗骨增止疼片中淫羊藿苷和芒柄花素的含量方法。方法:采用Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm)为色谱柱,以乙腈-水为流动相,线性梯度洗脱,检测波长为250 nm,流速为1.0 mL.min-1,柱温为30℃。结果:淫羊藿苷、芒柄花素进样量分别在0.2760-2.760μg(r=0.9999)和0.1378-1.378μg(r=0.9999)范围内与峰面积具有良好线性关系,平均回收率为98.58%(RSD=1.18%)和99.32%(RSD=0.41%)。结论:该方法准确,可靠,操作简便,可用于抗骨增止疼片中淫羊藿苷和芒柄花素的质量控制。Objective：To establish a HPLC method for simultanecus determination of icariin and formonone in kangguzeng Zhiteng tablets. Method：The separation was performed on the Shim-pack VP-ODS column （4.6mm×150mm, 5 μm） with acetonitrile-water as the mobile phase. The detection wavelength was at 250 nm, and the flow rate was 1.0 mL·min^-1. The column temperature was 30℃. Result：The linear response of icariin ranged from 0.2760μg to 2.760μg （r=0.9999）. The linear response of formonone ranged from 0.1378μg to 1.378μg （r=0.9999）. The average recovery rates of icariin were 98.58% （RSD=1.18%）. The average recovery rates of formonone were 99.32% ,RSD=0.41%. Conclusion：The method is accurate, reliable, simple, and can be used for the quality control of icariin and formonone in kangguzengzhiteng tablets.

关 键 词：抗骨增止疼片 高效液相色谱法 淫羊藿苷 芒柄花素 含量测定 

分 类 号：R283.6[医药卫生—中药学]