乌头根际土壤细菌群落多样性的PCR-DGGE分析  

PCR-DGGE analysis of bacterial community diversity in rhizosphere soils of Wutou

作　　者：吴清华[1] 王光志[1] 马云桐[1] 裴瑾[1] 康亚兰[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2014年第1期9-11,16,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(No.30901962)

摘　　要：目的:研究不同生长期乌头根际土壤微生物细菌群落结构的差异。方法:采用PCR-DGGE技术,研究不同产地不同健康状况的乌头根际土壤细菌群落结构的变化。结果:健康与患病乌头根际土壤细菌群落结构存在差异性,且患病植株具有能引起植物患病的病原种。结论:患病植株改变了乌头根际土壤的微生物细菌群落多样性,致病菌的存在导致了乌头植株的患病。根际土壤的健康状况对土壤微生物群落结构有显著影响,根际微生物群落结构的不同将导致植物对病原菌的抗性及品种差异。Objective： Study the structural differences of Wutou rhizosphere soil microbial bacterial communities in different growing season. Method： PCR-DGGE technology was applied to research the change of Wutou rhizosphere soil bacterial communities of different habitats and different health status of Wutou. Result： Difference can be detected between Wutou rhizosphere soil bacterial communities of health and illness Wutou, and diseased plants had pathogens which can cause illness of plants. Conclusion： Diseased plants change Wutou rhizosphere soil microbial bacterial communities, and existed pathogens induce the plant disease. The health status of the rhizosphere soil microbial community structure have significant impact on the rhizosphere microbial community structure differences which will lead to plant resistance to pathogens and species differences.

关 键 词：乌头 细菌 PCR—DGGE 群落结构 

分 类 号：R282.5[医药卫生—中药学]