Effect of Salinity Stress and Mutagenic Sodium Azide on Callus Induction and Plant Regeneration of Borage （Borago officinalis） in Vitro  

作　　者：AI-Mohammed Maher H. S. EI-Kaaby Ekhlas A. J AI-Anny Jenan A. Musa Abdul-kadhim J. 

机构地区：[1]Department of Genetic Engineering, Biotechnology Center, Baghdad 00964, Iraq [2]Ministry of Agriculture, Kufa 00964, Iraq

出　　处：《Journal of Life Sciences》2014年第8期660-667,共8页生命科学（英文版）

摘　　要：The effect ofmutagenic NaN3 at concentration （0.0, 2.0 and 4.0 mM） and salinity stress at the levels （6.5, 8.5, 10.5 and 12 dS/m） on the seeds germination, callus induction and plant regeneration fi＇om stressed callus of different borage explants were investigated. The results showed that （4.0 mM） NaN3 with 4 h incubation period increased germination percentage reached 83.3% rate. Moreover the responses of different explants for callus induction in the presence of different combinations from auxin and cytokines were varied and a combination of（2 mg/L ）from each of BA and IAA gave high callus fresh weight reached （229.6, 218.0, 237.3） for （shoot tips, cotyledon leaves and hypocotyls） respectively .Concerning to the effect of salinity stress on callus induction and plant regeneration a high reduction in callus flesh weight and plant regeneration were observed at the high level of NaCl.

关 键 词：Borage CALLUS SALINITY in vitro. 

分 类 号：S567.239[农业科学—中草药栽培] Q943.1[农业科学—作物学]