欧洲型PRRSV、PCV2二联重组痘苗病毒疫苗猪体免疫实验研究  被引量:6

Immunogenicity of a bivalent recombinant vaccine against European-type PRRSV and PCV2 in pigs

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作  者:韩继成[1] 任静强[1,2] 孙文超[1,3] 靖杰[1,4] 肖朋朋[1,4] 郭海宁[1,5] 陈兴[1,5] 鲁会军[1] 金宁一[1] 

机构地区:[1]中国人民解放军军事医学科学院军事兽医研究所,吉林长春130122 [2]中国农业科学院特产研究所,吉林长春130122 [3]广西大学动物科学技术学院,广西南宁530004 [4]吉林大学动物医学学院,吉林长春130062 [5]吉林农业大学动物科技学院,吉林长春130118

出  处:《中国病原生物学杂志》2014年第10期869-872,876,共5页Journal of Pathogen Biology

基  金:科技部863项目(No.2011AA10A208)

摘  要:目的评价实验室构建的欧洲型PRRSV和PCV2二联重组痘苗病毒猪体免疫效果。方法分别用重组痘苗病毒rVTT-PCV2-ORF2-EU-ORF3-ORF5和rVTT-EU-ORF3-ORF5进行猪体免疫试验,每周采血,分离血清,采用ELISA检测病毒特异性抗体、中和抗体检测及细胞因子水平,评价免疫效果。结果 rVTT-PCV2-ORF2-EU-ORF3-ORF免疫组35d时,猪血清欧洲型PRRSV GP3和GP5及PCV2ORF2特异性抗体,显著高于野毒组(t=6.61,t=9.21,t=10.34,P<0.05);猪血清中和抗体35d时,各免疫组均可产生较高的免疫水平,差异均无统计学意义(t=0.48,P>0.05);重组痘苗病毒疫苗rVV-ORF2-EU-ORF3-ORF5免疫可诱导IL-4和IFN-γ产生,增强猪体细胞免疫应答水平。结论欧洲型PRRSV、PCV2二联重组痘苗病毒疫苗能诱导猪体产生体液和细胞免疫应答,为欧洲型PRRSV、PCV2的新型疫苗研究奠定了实验基础。Objective To assess the immunological properties of a bivalent recombinant vaccine against European-type PRRSV and PCV2 in pigs. Methods Pigs were immunized with the recombinant vaccines rVTT-PCV2-ORF2-EUORF3-ORF5 and rVTT-EU-ORF3-ORF5.Blood was collected weekly and serum was separated to detect the level of virus-specific neutralizing antibodies and serum cytokines. Results On day 35,pigs immunized with rVTT-PCV2-ORF2-EU-ORF3-ORF5 had a higher level of antibodies specific to European-type PRRSV GP3 and GP5and PCV2ORF2 than pigs infected with wild-type viruses.Differences in levels of specific antibodies produced were statistically significant(t=6.61,t=9.21,t=10.34,P〈0.05).Detection of serum neutralizing antibodies indicated that immunized pigs had higher levels of antibodies on day 35.Differences in antibody levels were not statistically significant(t=0.48,P〉0.05).The recombinant vaccine rVV-ORF2-EU-ORF3-ORF5 induced IL-4and IFN-γ,enhancing the cellular immunity of pigs.Conclusion A bivalent recombinant vaccine against European-type PRRSV and PCV2 induced a better humoral response and cellular immune response,providing a new experimental basis for study of vaccines against European-type PRRSV and PCV2.

关 键 词:重组痘苗病毒rVTT-PCV2-ORF2-EU-ORF3-ORF 欧洲型PRRSV 免疫研究 

分 类 号:R373.21[医药卫生—病原生物学]

 

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