基于PGMY09/11引物的PCR法检测高危型HPV的效果观察  

Performance of PGMY09/11 primers-based PCR for detection of high-risk human papillomavirus(HPV)infection

作　　者：杜筱英 刘奇[2] 汤俊明[2] 赵彦平[2] 

机构地区：[1]无锡市惠山区人民医院,江苏无锡214005 [2]宜兴市人民医院,江苏宜兴214002

出　　处：《中国病原生物学杂志》2014年第10期932-935,共4页Journal of Pathogen Biology

基　　金：江苏省自然科学基金项目(No.BK2012564)

摘　　要：目的评估基于PGMY09/11引物的PCR法检测高危型人乳头瘤病毒(HPV)效果。方法采用HC II试剂盒和基于PGMY09/11引物的PCR技术检测感染HPV16的50份样品和感染HPV18的50份样品,样品模板DNA分别按照1∶1、1∶2、1∶4、1∶8和1∶16倍比稀释,比较2种方法检测的敏感性和一致性。同时采用HC II试剂盒和基于PGMY09/11引物的PCR技术检测感染单纯疱疹病毒(HSV)和沙眼衣原体(CT)的20份样品及单一感染HPV16和HPV18的10份样品,评估基于PGMY09/11引物的PCR检测HPV的特异性。结果 HC II试剂盒和基于PGMY09/11引物的PCR对1∶1、1∶2稀释的HPV16和HPV18的检出率均为100%;HC II对1∶4稀释的HPV16和HPV18的检出率为36%和46%,但不能检出1∶8和1∶16稀释的HPV16和HPV18样品;基于PGMY09/11引物的PCR对1∶4、1∶8和1∶16稀释的HPV16检出率分别为100%、90%和32%,对1∶4、1∶8和1∶16稀释的HPV18样品检出率分别为100%、86%和40%。HC II试剂盒和基于PGMY09/11引物的PCR对HPV16检测的整体一致性为84.1%(Kappa值为0.674),对HPV18检测的整体一致性为81.7%(Kappa值为0.598)。基于PGMY09/11为引物的PCR方法不能检测HSV和CT合并感染。结论基于PGMY09/11为引物的PCR方法检测出高危型HPV具有较高的敏感性和特异性,与市售HC II试剂盒检测结果具有较高一致性,可用于HPV感染诊断。Objective to evaluate the PGMY09/11 primer PCR method for detection of high-risk human papillomavirus（HPV）effect on. Methods HC II kit and detection based on PGMY09/11 primer PCR technologyof HPV16 infection and the infection of HPV18 in 50samples of 50 samples,sample template DNArespectively according to 1∶1,1∶2,1∶4,1∶8and 1to16 fold dilution,sensitivity of 2methods are compared and consistency.At the same time using HC II kit anddetection based on PGMY09/11 primer PCR technology of herpes simplex virus infection（HSV）and Chlamydia trachomatis（CT）in 10 samples of 20 samples and single infection of HPV16 and HPV18,to evaluate the specificity of the PCR detection based on the HPVPGMY09/11 primer.Results HC II kit and PGMY09/11 primers based upon the PCR 1∶1,1∶2dilution of HPV16 and HPV18detection rate was100%;the HC II on the 1∶4dilution of HPV16 and HPV18were 36% and 46%,but not detected in1：8and 1∶16dilution of HPV16 and HPV18samples;PGMY09/11 primers based on the PCR 1∶4,1∶8and 1：16dilution of HPV16 positive rates were100%,90%and32%,of 1∶4,1∶8and 1∶16dilution of HPV18 sample detection rates were 100%,86%and 40%.HC II kit and PGMY09/11 primer based PCR the overall consistency of HPV16 detection was 84.1%（Kappa=0.674）,the overall consistency of the HPV18 detection was 81.7%（Kappa=0.598）.Based on the PGMY09/11 method for detection of HSV primers can not PCR and CT. Conclusion Conclusion the specificity and sensitivity of PGMY09/11 based method for the primerPCR detection of high-risk HPV has higher,with the commercially available HC II kit for detecting results with higher consistency,can be used for the diagnosis of HPV infection

关 键 词：人乳头瘤病毒 HC II PCR 检测效能 

分 类 号：R373.9[医药卫生—病原生物学]