牙鲆致病性迟钝爱德华菌毒力基因的检测及序列分析  

Detection and Sequencing of Virulence Genes of Pathogenic Edwardsiella tarda in Paralichthys olivaceus

作　　者：朱文进[1] 陈翠珍[1] 苏咏梅[1] 葛慕湘[1] 张艳英[1] 靳晓敏[1] 房海[1] 

机构地区：[1]河北科技师范学院、河北省预防兽医学重点实验室,河北秦皇岛066600

出　　处：《动物医学进展》2014年第11期20-24,共5页Progress In Veterinary Medicine

基　　金：河北省现代农业产业技术体系特色海产品疫病防控与质量安全创新团队项目;河北省重点基础研究项目(№11960503D)

摘　　要：为探讨牙鲆致病性迟钝爱德华菌毒力基因携带与分布,以毒力基因为分子靶标研究其致病机理及建立快速检测方法,根据GenBank上的基因序列,设计2对引物扩增致病性迟钝爱德华菌毒力基因fimA和小肠结肠炎耶尔森菌毒力基因irp2,结果在7个供试牙鲆病例的130株致病性迟钝爱德华菌中,irp2毒力基因的阳性率为46.15%(60/130),fimA毒力基因的阳性率为100%,且均与已发表的参考菌株相应序列高度同源,同源性分别为97.32%和97.59%。可见耶尔森菌强毒力岛(HPI)在牙鲆致病性迟钝爱德华菌中广泛分布,但不同病例分离菌株间存在差异;fimA毒力基因存在于所有的被检致病性迟钝爱德华菌中,可以作为快速检测致病性迟钝爱德华菌的标志物。To investigate the carrying and distribution of the virulence genes in flounder paralichthys oliva-ceus,to study its pathogenesis and establish a rapid detection method as molecular targets,the pathogenic Edwardsiella tarda virulence genes fimA and theYersinia virulence genes irp2 were amplified,using the two pairs of primers designed according to GenBank gene sequences published.The results showed that the positive rate of irp2 gene was 46.15% （60/130）,the positive rate of fimA was 100%,in 130 Edwardsiella tarda strains of the 7 tested cases,and there were high homology with the sequences of corresponding ref-erence strains in the GenBank.The homologies were 97.32% and 97.59% respectively.TheYersinia HPI was widely distributed in pathogenic Edwardsiella tarda ,but there were differences among different strains isolated from the different cases.The virulence gene fimA was detected in all pathogenic Edwards-iella tarda ,and it can be used as rapid detection markers to detect the pathogenic Edwardsiella tarda .

关 键 词：牙鲆 迟钝爱德华菌fimA irp2 毒力基因 

分 类 号：S852.6[农业科学—基础兽医学]