三种核酸提取方法检测禽流感病毒的比较  

Comparison of Three Nucleic Acid Extraction Methods for Detection of Avian Influenza Virus

作　　者：张险朋[1] 董瑜 温贤诚[1] 徐振娜[1] 殷三鸿[1] 黄炳炽[1] 

机构地区：[1]东莞市动物疫病预防控制中心,广东东莞523086 [2]东莞市莞城兽医站,广东东莞523000

出　　处：《动物医学进展》2014年第11期116-119,共4页Progress In Veterinary Medicine

摘　　要：为比较不同核酸提取方法检测禽流感病毒敏感性的差异，将 H5N1禽流感抗原做10－2-10－10稀释，分别用 Trizol 法、磁珠法和膜吸附法提取上述抗原稀释度核酸模板，采用荧光 RT-PCR 对其进行评价。结果表明，在不同稀释度的抗原的检测中，3种核酸提取方法以膜吸附法提取效率最高，其依次为磁珠法、Trizol 法，经方差分析，3种核酸提取方法之间存在显著性差异（P＜0．05）。说明 3种核酸提取方法提取效率存在显著差异，核酸提取效率的高低影响禽流感病毒荧光 RT-PCR 检测的敏感性，建议实验室根据实际情况选择核酸提取的方法。To compare the efficiency of different nucleic acid extraction methods for detection of avian influ-enza virus.H5N1 avian influenza virus was used as template,Trizol,magnetic beads and membrane ad-sorption methods were used for nucleic acid extraction,H5N1 avian influenza virus were diluted from 10 -2 to 10-10 ,the efficiency of the three methods were evaluated through real-time RT-PCR.The variance anal-ysis showed there were significance differences among the three different methods（P〈0.05）.The mem-brane adsorption extraction method is the most efficient one,and followed by magnetic beads and Trizol extraction.The nucleic acid extraction efficiency is closely related to the sensitivity of the real-time RT-PCR results.The results suggested that the appropriate detection methods should be chosen for detection of clinical specimens based on actual situation.

关 键 词：禽流感病毒 核酸提取 提取效率 荧光RT-PCR 

分 类 号：S852.659.5[农业科学—基础兽医学]