丙型肝炎病毒抗体联合核酸定量检测对HCV早期诊断的价值  

Value of HCV antibody combined with nucleic acid quantitative detection in early diagnosis of HCV

作　　者：周昊[1] 田展飞[1] 樊和斌[1] 吴娟娟[1] 严福明[1] 

机构地区：[1]解放军161医院感染内科,武汉430010

出　　处：《西南国防医药》2014年第11期1179-1182,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨丙型肝炎病毒(HCV)抗体联合病毒核酸(HCV RNA)定量检测在丙型肝炎患者早期诊断中的价值。方法对我院收治的106例丙型肝炎病毒感染者血样分别测定HCV抗体、HCV RNA及丙氨酸氨基转移酶(ALT)。结果106份血样检测中,酶联免疫吸附试验HCV抗体检测阳性87例(82.1%),HCV金标法检测阳性69例(65.1%),HCV RNA检测阳性80例(75.5%),ALT阳性62例(58.5%)。HCV抗体检测阳性率明显高于HCV金标检测法(χ2=7.863,P<0.01)和ALT检测法(χ2=14.115,P<0.01);HCV抗体联合HCV RNA检测总阳性率为100.0%,明显高于HCV抗体联合金标法检测(83.0%)和HCV抗体联合ALT检测(90.6%)(χ2=19.670,P<0.01;χ2=10.495,P<0.01)。结论 HCV抗体联合HCV RNA检测可显著提高HCV的早期诊断率,减少漏诊,有利于HCV的早期诊断。Objective To study the value of hepatitis C virus（ HCV ）antibody combined with nucleic acid（ HCV RNA ） quantitative detection in early diagnosis of HCV. Methods Blood samples of the 106 patients with HCV receiving treatment in our hospital were taken to detect the HCV antibody,HCV RNA and alanine aminotransferase（ ALT）. Results In the detection of 106 blood samples,enzyme-linked immunosorbent assay for detection of HCV antibody showed that 87 ones were positive（ 82. 1%）,the gold standard method for detection of HCV showed that 69 ones were positive（65. 1%）,HCV RNA detection showed that 80 ones were positive（75. 5%）,and ALT detection showed that 62 ones were positive（58. 5%）. The positive rate of HCV antibody detection was significantly higher than that of HCV gold standard method（χ^2=7. 863,P〈0. 01）and ALT detection（χ^2=14. 115,P〈0. 01）. The total positive rate of HCV antibody combined with HCV RNA detection was 100. 0%,obviously higher than that of HCV antibody combined with the gold standard method（83. 0%）and HCV antibody combined with ALT detection（90. 6%）（χ^2=19. 670,P〈0. 01;χ^2=10. 495,P〈0. 01）. Conclusion HCV antibody combined with HCV RNA detection can significantly raise the early diagnostic rate of HCV,reduce misdiagnosis,and is conducive to the early diagnosis of HCV.

关 键 词：丙型肝炎 抗体 丙型肝炎病毒 早期诊断 丙氨酸氨基转移酶 

分 类 号：R512.6[医药卫生—内科学]