MAPK信号传导通路在人参多糖诱导K562细胞凋亡中的作用  

Role of MAPK Signal Transduction Pathway in Ginseng Polysaccharide Inducing K562 Cell Apoptosis

作　　者：罗英豪[1] 赖翔宇[1] 王霞[1] 尹偲偲 

机构地区：[1]重庆市肿瘤研究所药剂科,重庆400030

出　　处：《中国药业》2014年第21期18-19,共2页China Pharmaceuticals

摘　　要：目的探讨人参多糖诱导白血病细胞K562凋亡的机制。方法取对数生长期的K562细胞,调整密度为5×105个/m L,对照组予以常规培养;人参多糖（GPS）组加入400 mg/L GPS。采用流式细胞仪（MTT法）检测GPS对K562细胞增殖的影响;免疫细胞化学方法检测细胞中P-P38蛋白定位及表达量的变化。Western blotting检测细胞中P38及P-P38蛋白的变化。结果 GPS对K562细胞在体外有明显的增殖抑制作用,在100~800 mg/L质量浓度范围内,其抑制作用呈浓度依赖性,在400 mg/L时达到细胞的半数抑制率,且在48 h时抑制率达到高峰;免疫细胞化学显示,P-P38蛋白主要分布在胞浆,与对照组相比,P-P38蛋白表达明显增强,且向胞核转移。Western blotting检测结果显示P38总蛋白无明显变化（P〉0.05）;P-P38蛋白有增加趋势,且在作用48 h后增加明显（P〈0.05）。结论 GPS能促进K562细胞凋亡,其促凋亡机制可能是通过P38MAPK信号传导通路实现的。Objective To investigate the mechanism of ginseng polysaccharide（ GPS） inducing apoptosis of human leukemia K562 cells.Methods The density of K562 cells with logarithmic phase was regulated to 5 × 105/ m L. The blank control group was performed the routine culture; the GPS group was added with 400 mg / L GPS. The effects of GPS on the proliferation of K562 cells were determined by MTT assay; the localization and expression quantity change of P38 were detected by the immunohistochemistry method and the P-P38 protein change was detected by Western blot. Results GPS significantly inhibited the K562 cells proliferation in vitro in a dose dependent manner during the concentration range of 100- 800 mg / L,which at the concentration of 400 mg / L reached the cellular half number inhibiting rate and the inhibiting rate reached its peak at 48 h; the immunocytochemistry results showed that P- P38 was mainly distributed in cytoplasm,compared with the blank group,the P- P38 expression was significantly enhanced and shifted to the nuclear. The Western blot results showed that the total protein of P38 had no significant changes（ P〉0. 05）; P- P38 had an increasing trend and was increased significantly after 48 h action（ P〈0. 05）. Conclusion GPS promotes the apoptosis of K562 cells,its mechanism may be realized by the P38 MAPK signal transduction pathway.

关 键 词：P38MARK信号传导通路 人参多糖 K562细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]