复方天麻止痛颗粒质量标准研究  

Study on Quality Standard of Compound Gastrodia Elata Analgesic Granules

作　　者：任卫琼[1] 李珊[1] 陈迎春[1] 欧阳荣[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国药业》2014年第21期35-37,共3页China Pharmaceuticals

摘　　要：目的制订复方天麻止痛颗粒的质量标准。方法采用薄层色谱（TLC）法鉴别方中川芎、桃仁、赤芍及红花;采用高效液相色谱法（HPLC）测定天麻素的含量,色谱条件为Hyperisil ODS C18柱（200 mm×4.6 mm,5μm）,流动相为甲醇-0.1%磷酸（11∶89）,流速1.0 m L/min,检测波长222 nm,柱温30℃。结果 TLC色谱中斑点清晰,易于识别。HPLC法精密度、重现性良好,天麻素进样量在0.08~0.44μg范围内与峰面积具有良好的线性关系,平均回收率为98.13%,RSD=0.77%（n=6）。结论该方法准确、可靠,可有效控制复方天麻止痛颗粒的质量。Objective To formulate the quality standard of Compound Gastrodia Elata Analgesic Granules. Methods The TLC method was adopted to identify the effective components in this preparation. The gastrodin content was detected by HPLC. The chromatographic conditions were the Hyperisil ODS column（ 200 mm × 4. 6 mm,5 μm）,the mobile phase of methanol- 0. 1% phosphoric acid（ 11 ∶ 89）,a flow rate of 1. 0 m L / min,the detection wavelength of 222 nm and the column temperature of 30 ℃. Results The TLC spots were clear and easy to be recognized; the HPLC method had good precision and good reproducibility. The linear range of gastrodin was 0. 08-0. 44 μg,the average recovery rate was 98. 13%, RSD = 0. 77%（ n = 6）. Conclusion This method is accurate,reliable and can effectively control the quality of Compound Gastrodia Elata Analgesic Granules.

关 键 词：复方天麻止痛颗粒 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]