反相高效液相色谱法测定暖宫孕子丸浸膏中黄芩苷含量  

Determination of Baicalin Content in Nuangong Yunzi Pill Extract by RP- HPLC

作　　者：田静 许亚玲 任汝仙[3] 

机构地区：[1]贵州省药品集中采购服务中心,贵州贵阳550002 [2]贵州省药品检验所,贵州贵阳550004 [3]贵州省遵义市第一人民医院,贵州遵义563002

出　　处：《中国药业》2014年第21期49-50,共2页China Pharmaceuticals

摘　　要：目的建立测定暖宫孕子丸浸膏中黄芩苷含量的反相高效液相色谱法。方法以70%甲醇为溶剂,加热回流30 min提取。采用C18柱（150 mm×4.6 mm,5μm）为分析柱,流动相为甲醇-0.4%磷酸（50∶50）,检测波长为280 nm,柱温30℃。结果黄芩苷的进样量在0.057 147~0.571 47μg范围内与峰面积线性关系良好（r=0.999 9）;平均回收率为100.57%,RSD为1.21%（n=6）。结论该方法快速简便、准确可靠,重现性好,结果稳定,可用于暖宫孕子丸浸膏中黄芩药材的质量控制。Objective To establish the quantitative analysis method for determining the content of baicalin in Nuangong Yunzi Pill Extract by RP- HPLC so as to provide the quality standard for the medicinal material in this preparation. Methods With 70% methanol as solvent, the extraction was conducted by heating reflux for 30 min. The C18 column（ 150 mm × 4. 6 mm, 5 μm） was adopted with methanol- 0. 4% phosphoric acid（ 50 ∶ 50） as the mobile phase; the detection wavelength was 280 nm and the column temperature was30 ℃. Results Under the above chromatographic conditions, the injection volume of baicalin in the range of 0. 057 147- 0. 571 47 μg（ r = 0. 999 9） showed a good linear relationship with the peak area; the average recovery rate of baicalin was 100. 57%, RSD = 1. 21%（ n = 6）. Conclusion This method is rapid, simple, accurate and reliable with good reproducibility and stable results and can be used for the quality control of Radix Scutellariae medical material in Nuangong Yunzi Pill Extract.

关 键 词：黄芩苷 暖宫孕子丸浸膏 质量控制 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]