联合检测支气管肺泡灌洗液中肿瘤坏死因子α及人转化生长因子β1的临床意义  

Clinical significance of joint detection of tumor necrosis factor α and transforming growth factor β1 in bronchial alveolar lavage fluid

作　　者：于露[1] 黄江[1] 刘跃建[1] 

机构地区：[1]四川省医学科学院.四川省人民医院呼吸内科,四川成都610072

出　　处：《实用医院临床杂志》2014年第6期37-38,共2页Practical Journal of Clinical Medicine

基　　金：四川省卫生厅科研基金资助项目(编号:090410)

摘　　要：目的探讨结缔组织疾病与其他病因所致间质性肺疾病支气管肺泡灌洗液(BALF)中肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和转化生长因子β1(transform growth factorβ1,TGF-β1)的差异。方法 2010年6月至2011年5月在我院诊治的13例结缔组织疾病所致间质性肺疾病(interstitial lung disease with connective tissue disease,CTD-ILD)患者(CTDILD组)、12例其他病因所致的间质性肺疾病(NCTD-ILD)患者(NCTD-ILD组)和21例同期健康体检者(对照组),均采用ELISA法测定BALF中TNF-α及TGF-β1水平。结果 CTD-ILD组和NCTD-ILD组两项因子均明显高于对照组,且CTD-ILD组高于NCTD-ILD组,差异均有统计学意义(P<0.05)。结论 BALF中TNF-α及TGF-β1水平差异有助于间质性肺疾病的诊断,两项因子联合检测对于CTD-ILD的鉴别诊断亦有提示意义。Objective To investigate the difference of tumor necrosis factor α （TNF-α） and transform growth factor β1 （ TGF- β1 ）in bronchial alveolar lavage fluid （BALF）between patients with connective tissue disease induced interstitial lung disease （CTD- ILD） and non connective tissue disease induced interstitial lung disease （NCTD-ILD）. Methods The BALF levels of TNF-α and TGF- β-1 in 13 cases with CTD-ILD （group 1 ）, 12cases with NCTD-ILD （group 2）and 21 healthy controls （group 3 ）were determined with specific enzyme-linked immunosorbent assays （ELISA）. Results The levels of TNF-α and TGF-β1 in the group 1 and 2 were higher than that in the group 3 （ P 〈 0. 05 ）, and the levels of the two factors in the group 1 was significantly higher than that in the group 2 （ P 〈 0. 05）. Conclusion The determination of TNF-α and TGF-β1 in BALF contributes to the diagnosis of interstitial lung disease and helps to the differential diagnosis of CTD-ILD.

关 键 词：肿瘤坏死因子Α 人转化生长因子β1 支气管肺泡灌洗液 结缔组织疾病 间质性肺疾病 

分 类 号：R619.6[医药卫生—外科学]