云南主栽甘蓝型油菜品种的遗传多样性分析与分子鉴定  

Genetic Diversity Analysis and Molecular Identification of Brassic napus Varieties Mainly Planting in Yunnan Province

作　　者：李淑娟[1] 刘岱[2] 孙桂林[1] 彭少丹[3] 陈升位[1] 王勤方 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]昆明医科大学图书馆,云南昆明650201 [3]云南农业大学农科专业基础实验教学中心,昆明650201

出　　处：《种子》2014年第11期27-31,共5页Seed

基　　金：云南省农业厅"油菜产业技术体系建设";云南省科技厅"官春云院士工作站"项目经费资助

摘　　要：利用SSR分子标记技术,对9份来自云南的主栽甘蓝型油菜品种进行遗传多样性及其分子鉴定分析。共获得146个SSR标记位点,其中有119个标记位点具有多态性,多态率达81.5%。遗传多样性分析表明,云南主栽甘蓝型油菜品种具有较高的遗传多样性,其有效等位基因数为1.422 8,平均期望杂合度(He)为0.248 2,平均Shannon多样性指数为0.371 1,多态位点百分率为68.91%,花油8号与花油3号之间的遗传相似系数最大,也只有0.831 9,在遗传相似系数为0.72时,9份甘蓝型油菜分别被分成了4个类群,并获得了能够区分云南9个品种的分子标记CB 10364。The genetic diversity and molecular identification of 9 Brassica napus varieties from Yunnan wasanalyzed by SSR markers. The results showed that 146 alleles were obtained and that its 119 alleles werepolymorphic, that the polymorphic rate was 81.5%. Furthermore, the effective number of alleles was 1. 422 8,the Nei＇ s gene diversity （He） was 0.248 2, the Shannon＇ s information index （I） was 0.371 1, and the percent-age of polymorphic loci （PPL） was 68.91%. The genetic similarity coefficient between huayou 8 and huayou 3was biggest,only 0. 831 9. The 9 rapeseed varieties could be divided into 4 groups at the level of 0.72 geneticsimilarity coefficient. 9 Brassic napus varieties could be identified by CB 10364.

关 键 词：甘蓝型油菜 遗传多样性 分子标记 云南 

分 类 号：Q946[生物学—植物学]