外援NO对扁蓿豆种子萌发期抗旱性的影响  

The Effect of NO on Drought Resistance During Medicago ruthenica Seed Germination

作　　者：景媛媛[1] 鱼小军[1] 徐长林[1] 满元荣[1] 岳生福 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃省草业工程实验室,兰州730070

出　　处：《种子》2014年第11期38-42,共5页Seed

基　　金：甘肃省高校科研业务费

摘　　要：以扁蓿豆种子为试验材料,采用PEG(聚乙二醇,分子量6000)模拟干旱处理,研究了一氧化氮(NO)供体硝普钠(SNP)缓解干旱胁迫对扁蓿豆(Medicago ruthenica)种子萌发和幼苗生长的影响。结果表明,在-0.6 MPa的PEG模拟干旱胁迫下,扁蓿豆种子的发芽率、发芽势、发芽指数、活力指数均较对照呈下降趋势。低浓度的SNP(0.01 mmol/L)对PEG干旱胁迫下扁蓿豆种子的发芽率和发芽势的缓解作用最强,SNP浓度为0.05 mmol/L时,扁蓿豆种子发芽指数、活力指数最高,幼苗根长和芽长最大。经过外源NO处理可以显著地增加扁蓿豆幼苗的生物量,SNP浓度为0.05 mmol/L,0.1 mmol/L及1.0 mmol/L时,可以显著增加扁蓿豆幼苗鲜重;SNP浓度较低(0.01 mmol/L)时,幼苗干重增加。The effects of NO on drought resistance of Medicago ruthenica seed germination by using differenttreatments included 0 mmol/L SNP,PEG,0.01 mmol/L SNP ＋ PEG,0.05 mmoL/L SNP ＋ PEG,0.1 mmol/LSNP ＋ PEG,0.5 mmol/L SNP ＋ PEG, 1.0 mmol/L SNP ＋ PEG. The results showed that under stress of PEGdrought of -0.6 MPa,Medicago ruthenica seed germination rate, germination potential, germination index andvigor index decreased compared with the control. Low concentrations of SNP （0. 01 mmol/L ） have thestrongest alleviation for PEG simulated drought stress of Medicago ruthenic seed germination rate andgermination potential. When the concentration of SNP is 0. 05 mmol/L, the germination, vigor index ofMedicago ruthenic seed is the highest, root length and shoot of the seedling is the largest, growing withexcellent. Exogenous NO can significantly increase seedlings biomass of Medicago ruthenic, whenconcentrations of SNP is 0.05 mmol/L, 0. 1 mmol/L and 1. 0 mmol/L, it can increase fresh weightsignificantly ; when concentrations of SNP is lower （0.01 retool/L）, dry weight of seeding increase.

关 键 词：一氧化氮 扁蓿豆 PEG 干旱胁迫 萌发 

分 类 号：S543[农业科学—作物学]