基于物质基础和抗脑缺血作用的芎汤加味方提取工艺研究  

Research of Extraction Process of Modified Xiongqiong Decoction Based on Material Basis and Effect of Anti-Cerebral Ischemia

作　　者：冯群[1,2] 迟雪洁[3] 张亚囡[4] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]天津中医药大学,天津300073 [3]潍坊市中医院,山东潍坊261041 [4]济宁市药品检验所,山东济宁272000

出　　处：《中国药物警戒》2014年第11期641-644,共4页Chinese Journal of Pharmacovigilance

基　　金：重大新药创制专项:中药复方药理学研究及药效学评价关键技术(2009ZX09502-015)

摘　　要：目的在小鼠全脑缺血再灌注损伤模型的基础上,根据芎汤加味方所含物质基础研究进展,进行其提取工艺研究。方法结合文献进行芎汤加味方6个制备工艺的研究,测定各工艺提取物中有效成分的含量,并观察各个提取物对小鼠全脑缺血再灌注损伤模型的保护作用。通过物质基础的含量测定和抗脑血活性的比较,筛选最有效的提取工艺。结果通过挥发油、核桃油、阿魏酸提取率指标进行综合评价,工艺5提取物的有效成分保留相对全面,对小鼠全脑缺血和缺氧损伤程度的改善作用最强。结论采用"质量-药效"综合评价模式可保障中药复方的有效性。初步确定了工艺5为芎汤加味方的最佳提取工艺,其具体优化参数有待于进一步研究。Objective Extraction process of modified Xiongqiong decoction was researched and optimized on the basis of mice models of cerebral ischemia-reperfusion damages according to its material basis. Methods Six extraction processes of modified Xiongqiong decoction were explored according to modern references. Contents of active ingredients in the extracts were determined and protective effects of them on mice models of cerebral ischemia-reperfusion damages were observed. Optimum extraction process was selected according to determination of material basis and comparison of anti-cerebral ischemia. Results Active ingredients in the extract of the fifth extraction process is comprehensive relatively according to the integrated evaluation to volatile oil, walnut oil and ferulic acid. Extract of the fifth extraction process improved most obviously cerebral ischemia-reperfusion damages in all the extracts. Conclusion Model of＆quot;quality-efficacy＆quot; comprehensive assessment can guarantee effectiveness of Chinese medicine compound. The fifth extraction process has been confirmed to be optimum extraction process of modified Xiongqiong decoction preliminarily. However specific optimum parameters of the fifth extraction process need to be confirmed by a further study.

关 键 词：芎汤加味方 全脑缺血再灌注损伤 提取工艺 含量测定 

分 类 号：R965[医药卫生—药理学]