禾本科牧草胚性愈伤组织诱导及再生研究进展  

Progress in Embryogenic Callus Induction and Regeneration Research of Gramineous Forage

作　　者：李达旭[1] 张玉[1] 刘刚[1] 白史且[1] 鄢家俊[1] 游明鸿[1] 

机构地区：[1]四川省草原科学研究院,四川成都611731

出　　处：《草业与畜牧》2014年第5期53-57,62,共6页Pruataculture & Animal Husbandry

基　　金："十二五"国家科技支撑计划项目(2011BAD17B03);四川省草种质资源平台建设项目资助

摘　　要：禾本科牧草在草畜产业发展和生态环境保护等方面起着至关重要的作用。近年来,随着生物技术的发展,国内外在牧草胚性愈伤组织诱导和再生研究方面取得了较大进展。该文在归纳总结该领域研究成果的基础上,对影响禾本科牧草胚性愈伤组织诱导和再生的几个主要因素进行了探讨。大量研究结果表明,植物激素类型和供试材料的基因型是影响胚性愈伤组织诱导和再生效率的最主要因素。培养基中添加适量的水解酪蛋白或多种氨基酸不但可以提高胚性愈伤组织的诱导效率,还可提高愈伤组织的质量。同时对禾本科牧草胚性愈伤组织诱导和再生的应用前景进行了展望。Gramineous Forage are critical to sustainable livestock and environmental protection. In recent years, with the rapid development of biotechnology, significant progress has been made in induction and regeneration of grasses embryogenic callus. This review describes the development of induction and regeneration for gramineous forage embryogenic callus and summarizes the latest progress of some important influencing factors on embryogenic callus induction and regeneration. The geno type of plant hormones and the test material type are the most important factor affecting callus induction and regeneration efficiency. Medium, add the appropriate amount of hydrolyzed casein or more amino acids can not only improve the efficiency of embryogenic callus induction, but also improve the quality of callus. We summarize the methods of embryogenic callus induction and techniques of embryogenic callus regeneration and point out prospects for further research into callus induction and regeneration of in forage

关 键 词：禾本科牧草 胚性愈伤组织 诱导 再生 

分 类 号：Q813[生物学—生物工程]