两种脂肪细胞内脂滴染色方法的比较研究  

Comparative research of two methods for staining lipid droplet in adipocytes

作　　者：秦红霞[1] 赵玲[1] 王宇豪[2] 宁椿游 罗毅[2] 

机构地区：[1]四川农业大学动物医学院 [2]四川农业大学动物科技学院,雅安625014

出　　处：《中国组织化学与细胞化学杂志》2014年第6期544-547,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的比较油红O染色法和尼罗红染色法对脂肪细胞内脂滴染色的优劣,探讨科研工作中更优的脂滴染色方法。方法采用经典的"鸡尾酒"法诱导3T3-L1前脂肪细胞分化;诱导分化8d后,对细胞内脂滴进行油红O染色和尼罗红染色,观察脂滴形态并测定甘油三酯含量。结果两种染色方法中甘油三酯的测定结果一致。但油红O染色过程较为繁琐、耗时较长,染料不易清洗干净,导致染料残留,从而引入实验误差,影响测定结果;而尼罗红荧光染色操作简单、耗时短以及背景更洁净。结论尼罗红荧光染色法应用于脂肪细胞内脂滴的鉴定和定量测定更加简单、便捷。Objective To compare oil red O staining with Nile red staining,and explore a better staining method for lipid droplets.Methods Differentiation of 3T3-L1pre-adipocytes was induced by a classical cocktail method.After induction of differentiation for 8days,the morphology of lipid droplets and content of intracellular triglyceride were detected by oil red O staining and Nile red staining.Results The results of the two staining methods were identical.But oil red O staining process was more complicated and timeconsuming while the dye was not easily cleaned,which may result in experimental error and affect the result.In contrast,Nile red staining had the advantage of simple operation,short time and clean background.Conclusion Nile red fluorescent staining is simpler and more accurate for the identification and quantitative determination of lipid droplets.

关 键 词：脂肪细胞 脂滴 油红O 尼罗红 

分 类 号：Q2-33[生物学—细胞生物学]