新疆陆地棉遗传连锁图谱构建及叶绿素含量和光合速率的QTL定位  

Construction of Linkage Genetic Map and QTL Mapping for the Chlorophyll Content and Photosynthetic Rates in Upland Cotton

作　　者：郑巨云[1] 龚照龙[1] 王俊铎[1] 李雪源[1] 艾先涛[1] 梁亚军[1] 吐尔逊江[1] 多力坤[1] 莫明[1] 

机构地区：[1]新疆农业科学院经济作物研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2014年第9期1577-1582,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金(青年基金)(2011211B43);新疆维吾尔自治区农作物细胞工程重点实验室开放项目(XJYS0302-2011-03)

摘　　要：【目的】研究新疆陆地棉叶绿素含量和光合速率的QTL定位,为陆地棉高光效分子标记辅助选择育种提供理论指导。【方法】以高光效的陆地棉品系ZX1789和低光效陆地棉品系E563号组配衍生的(ZX1789×E563)F2和F2∶3家系为材料,应用复合区间作图法,对F2∶3家系的叶绿素含量、光合速率进行QTL定位。【结果】构建了包括9个连锁群、标记间平均距离14.7 cM、全长为587.3 cM的遗传连锁图谱,约占棉花基因组的11.6%。检测到1个与叶绿素含量相关的QTL,位于第19号染色体,可解释表型变异的21.25%,检测到2个与单叶光合速率相关的QTLs,分别位于第9、20号染色体,可解释表型变异的8.39%、14.52%。【结论】检测到与陆地棉叶绿素含量、光合速率性状相关的QTL标记位点,有利于新疆陆地棉高光效辅助选择育种,为进一步提高育种效率打下基础。[Objective] This research attempts to provide theoretic guidance in high photosynthetic efficiency molecular marker-assisted breeding of cooton by QTL mapping for the chlorophyll content and photosynthetic rates in Xinjiang upland cotton.[Method] In this paper,a genetic linkage map was constructed by 180 F2 segregation population derived from a cross between ZX1789 × E56.The chlorophyll content and photosynthetic rates of the F2 plants and the F2∶3 family lines were analyzed by composite interval mapping （CIM）.[Result] This map included 41 markers distributing in 9 linkage groups,covering 587.3 cM,accounting for 11.6% of the cotton genome,and with an average distance of 14.7 cM between two markers.These QTLs were located on 3 chromosomes,which were Chr.19,Chr.9,and Chr.20 and explained their corresponding phenotypic variations with 21.25%,8.39%,and 14.52%.Additive effects were mainly responsible for heredity of two characters.[Conclusion] These QTLs might provide valuable theoretical guidance for marker-assisted selection（MAS） in cotton high photosynthetic rates breeding program.

关 键 词：棉花 超高产 光合特性 冠层 

分 类 号：S526[农业科学—作物学] S503.52