库尔勒香梨上苹果茎沟病毒的三种分子生物学检测技术比较研究  

Comparative Study of Three Kinds of Molecular Biology Detection Technology of Apple stem grooving Virus from Korla Pear

作　　者：黄妍妍[1] 陈小飞[1] 朱天生[1] 

机构地区：[1]塔里木大学植物科学学院,新疆阿拉尔843300

出　　处：《塔里木大学学报》2014年第3期10-15,共6页Journal of Tarim University

基　　金：塔里木大学校长基金硕士项目(TDZKSS201215);国家自然科学基金项目(31060238)

摘　　要：采用反转录聚合酶链式反应(RT-PCR)、试管捕捉反转录聚合酶链式反应(TC-RT-PCR)及免疫捕捉反转录聚合酶链式反应(IC-RT-PCR)技术对20份库尔勒香梨样品进行苹果茎沟病毒(Apple stem grooving virus,ASGV)检测,结果显示RT-PCR检测出7份样品感染ASGV,再使用TC-RT-PCR与IC-RT-PCR进行复检,有6份样品检测到ASGV,说明TCRT-PCR与IC-RT-PCR不如RT-PCR的灵敏度高,但是TC-RT-PCR与IC-RT-PCR不需要提取总RNA,操作简便,且减少有毒有机溶剂的危害与污染。TC-RT-PCR与IC-RT-PCR相比,检测得到的特异性带较清晰。In this study 20 Apple stem grooving virus samples from Korla Pear were detected using reverse transcription polymerase chain reaction（ RT-PCR）,tube capture reverse transcription polymerase chain reaction（ TC-RT-PCR） and Immunocapture reverse transcription polymerase chain reaction（ IC-RT-PCR）,the results showed that there were 7 samples infected with Apple stem grooving virus were detected by RT-PCR,then re-inspected the 7 samples with TC-RT-PCR and IC-RT-PCR,and 6 samples were detected with Apple stem grooving virus. While the sensitivity of RT-PCR was higher than those of TC-RT-PCR and IC-RT-PCR,the TC-RT-PCR and IC-RT-PCR were simply operated with no need extracting the total RNA and reducing the harm and pollution of toxic organic solvents. The obtained specific fragments of TC-RT-PCR was more clearly which compared with IC-RT-PCR.

关 键 词：RT-PCR TC-RT-PCR IC-RT-PCR 苹果茎沟病毒 库尔勒香梨 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]