基于光诱导荧光的维生素K1检测方法研究  

Determination of vitamin K1 based on photo-induced fluorescence

作　　者：李云云[1] 章竹君[1] 马红燕[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《安徽大学学报（自然科学版）》2014年第5期78-84,共7页Journal of Anhui University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30872371);陕西省重点实验室资助项目(12JS119)

摘　　要：建立一种灵敏度高、选择性好的基于紫外光诱导荧光的维生素K1（VK1）检测方法.不发荧光的VK1经紫外光（254 nm,15 W）照射后可转变为具有很高荧光量子产率的光化产物,检测出其特征荧光峰在465nm处;当加入咪唑并经光诱导后,光化产物荧光特性发生变化,在330 nm处又出现一新的较强荧光发射峰,两峰间的stokes位移长达135 nm.同时发现,随着VK1浓度的增加,465 nm处的荧光峰强度增加,而330 nm处的峰强度反而减小.方法的检出限为0.003 μg· mL-1（S/N=3）,对1.0 μg·mL-1VK1溶液进行11次平行测定,相对标准偏差（RSD）为2.4％.该法用于注射液及人血清中VK1含量测定,回收率为97％～104％,结果令人满意.A highly sensitive and selective photo induced fluorescence （PIF）method was developed for the determination of vitamin K1（VK1）.The method was based on ultraviolet （UV） irradiation （254 nm,15 W） of non-fluorescent VK1 to produce an intensively fluorescent product.The fluorescent photoproduct generated showed native fluorescence with emission maxima at 465 nm.When adding imidazole,the fluorescence characteristics of photoproducts produced from UV irradiation of VK1 were changed.Consequently,a new strong emission peak appeared at 330 nm and the stokes shift was 135 nm between the two emission peaks.In addition,when increasing the concentration of VK1,the fluorescence signal increased at 465 nm,but reduced at 330 nm.The detection limit （signal-to-noise ratio =3） was 0.003 μg · mL-1 （S/N=3） and the relative standard deviation was 2.4％.The method also seems to be suitable for human serum analysis providing satisfactory recoveries （97％-104％）.

关 键 词：荧光 紫外光诱导 咪唑 维生素K1 血清 注射液 

分 类 号：O657.39[理学—分析化学] Q568[理学—化学]