转hFat-1基因猪外源基因整合分析及拷贝数测定  

Analysis of Exogenous Gene Integration and Copy Number in hFat-1 Transgenic Pigs

作　　者：华文君[1] 华再东[1] 郑新民[1] 毕延震[1] 肖红卫[1] 张立苹[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《江西农业学报》2014年第11期99-101,共3页Acta Agriculturae Jiangxi

基　　金：转基因生物新品种培育重大专项(2011ZX08010-3;2014ZX08006-003);国家自然科学基金项目(31201790);湖北省农业科技创新中心项目(2011-620-001-003)

摘　　要：对转人源化Fat-1基因猪的15头传代仔猪进行PCR检测,发现有7头阳性猪,其断奶后成活5头仔猪。对这5头仔猪进行Southern印迹杂交试验,确认3头仔猪整合有hFat-1基因。采用荧光实时定量PCR法测定原代转hFat-1基因公猪及3头阳性仔猪外源基因的拷贝数,分别是2、1、1、2个拷贝。转hFat-1基因猪能够将外源基因遗传到下代仔猪,但外源基因具有遗传不稳定的趋向。Fifteen heads of hFat-1 transgenic offspring piglets were detected by PCR, 7 heads of piglets were positive, and 5 heads of piglets survived after weaning.Southern blotting cross experiment confirmed that the exogenous gene hFat-1 had been integrated into 3 piglets among these 5 piglets.The copy numbers of hFat-1 in primary hFat-1 transgenic boar and 3 positive piglets were detected by fluorescence real-time Q-PCR, and they were 2, 1, 1, and 2 copies respectively.The integration of exogenous gene in hFat-1 transgenic pigs could be inherited to F1 -generation piglets, but could not be stably inherited.

关 键 词：猪 转hFat-1基因 整合 拷贝数 

分 类 号：Q785[生物学—分子生物学]