火龙果炭疽病病原鉴定与ITS序列分析  

Identification and ITS Sequence Analysis of Pitaya Colletotrichum gloeosporioide

作　　者：郑伟[1] 王彬[1] 彭丽娟[2] 李兴忠[1] 

机构地区：[1]贵州省果树科学研究所,贵州贵阳550006 [2]贵州大学,贵州贵阳550000

出　　处：《西南农业学报》2014年第5期1970-1973,共4页Southwest China Journal of Agricultural Sciences

基　　金：贵州省重大科技专项"火龙果产业化关键技术研究与集成示范"子课题[黔科合重大专项字(2012)6006];农业科技成果转化资金项目"火龙果新品种紫红龙转化与示范"(2012GB2F20040);贵州省科技创新人才团队建设项目"贵州省精品火龙果研究科技创新团队"[黔科合人才团队(2013)4005];贵州省农业科学院院专项"火龙果病虫害调查及防治技术研究"[黔农科院院专项(2008)013]

摘　　要：为对火龙果炭疽病病的防治提供理论依据,对从火龙果炭疽病病株上分离得到的病原菌进行形态特征、致病性以及核糖体DNA.ITS序列分析。结果表明,引起火龙果炭疽病的炭疽菌有2种,一种分生孢子为镰刀形,该菌在PDA培养基上PDA培养基上菌落生长圆形,呈莲花状;另一种分生孢子为椭圆形,PDA培养基上菌落生长圆形。克隆分析菌株的核糖体DNA.ITS区域序列,弯孢病原菌与C.truncatum的菌株同源性高达100%,直孢病原菌与C.gloeosporioides的菌株同源性高达100%,结合形态学鉴定引起火龙果炭疽病病原菌为半知菌亚门腔孢纲黑盘孢目炭疽菌属(Colletotrichum)的C.truncatum和C.gloeosporioides。Pathogen isolates causing pitaya C.gbeosporioide were identified by morphological characteristics,pathogenicity and the sequence of ribosmal DNA-ITS to provide a theoretical reference for prevention and control of pitaya C.gloeosporioide.Results：there were two colletotrichum species causing pitaya C.gloeosporioide.one was sickle shaped,the bacteria in PDA culture medium was growing round,a lotus shaped,another anthrax spores was oval,PDA medium for colony growth circle.Ribosomal DNA.ITS region sequence analysis of cloned strains indicated that Curvularia pathogens and C.truncatum strains had as high as 100 ％ homology,straight spore pathogen and C.gloeosporioides strains had 100 ％ homology,combined with morphological identification,pathogenic bacteria causing pitaya C.gloeosporioide was C.truncatum and C.gloeosporioides of the genera Colletotrichum.

关 键 词：火龙果 炭疽病 rDNA-ITS序列 鉴定 

分 类 号：S668.9[农业科学—果树学]