28个果桑品种(系)的遗传多样性ISSR分析  

Analysis on Genetic Diversities of 28 Fruit Mulberry Varieties (Strains) by ISSR

作　　者：黄盖群[1] 佟万红[1] 危玲[1] 殷浩[1] 何宁佳 刘刚[1] 郑继川[1] 姚永权[1] 李永远[1] 

机构地区：[1]四川省农业科学院蚕业研究所,四川南充637000 [2]家蚕基因组学国家重点实验室,重庆400716

出　　处：《西南农业学报》2014年第5期2095-2100,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省青年科技基金项目(2014JQ0026);四川省应用基础研究项目(2012JY0130);四川省财政基因工程青年基金专项(2012QNJJ-020);四川省"十二五"农作物育种攻关项目(2011yzgg-13-01-02);四川优势特色农业产业科技创新与示范工程建设项目(2013XXXK-016);"十二五"四川省财政育种基因工程项目(2011JYGC10-027-02);四川省农业科学院"青年科研骨干"访问学者

摘　　要：为分析果桑品种（系）素缘关系,拓展果桑种植资源创新利用新途径.本研究对28份果桑品种（系）进行遗传多样性ISSR分析,结果表明,筛选出的12个通用引物共扩增出68条清晰的条带,其中多态性条带61条,占89.5％,平均每个引物扩增出多态性条带5.6条;遗传相似系数表明,28个资源间遗传相似系数变化范围为0.2741 ～0.8529;从聚类分析结果来看,可将全部供试材料严格地分为8类,且种间的遗传相似性差异较大.To identify genetic relationship among fruit mulberry varieties（strains） and expand innovation and utilization of germplasm resource,the genetic diversties of 28 fruit mulberry reeources were analyzed by ISSR molecular marker.The result showed that 68 clear bands were amplified by 12 universal primers,including 61 polymorphic bands which accounted for 89.5 ％,with average 5.6 amplification polymorphic bands of each primer.The genetic similarity coefficieets between 28 resorees ringed from 0.2741 to 0.8529.The results of cluster analysis indicated that all materials could be divided into 8 claases,and there was significantly different at the genetic similarity between species.

关 键 词：果桑 遗传多样性 ISSR 亲缘关系 

分 类 号：S663[农业科学—果树学]