橡胶树AtSAG12同源基因的克隆与表达特性分析  

Molecular Cloning and Expression Analysis of Arabidopsis SAG12 Homologue from Rubber Tree (Hevea brasiliensis Muell. Arg.)

作　　者：邹智[1] 刘建汀 庄美露[1] 杨礼富[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/儋州热带作物科学观测实验站,海南儋州571737

出　　处：《西南农业学报》2014年第5期2168-2172,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学资金(31371556);海南省自然科学基金(312026);中国热带农业科学院橡胶研究所基本科研业务费专项(1630022011014)

摘　　要：本研究以橡胶树为研究对象,采用PCR技术从其根组织中分离到一条长1048 bp的cDNA,并对其进行序列分析。结果表明,该序列包含一1023 bp的开放读码框(ORF),13 bp的5’UTR和12 bp的3’UTR;ORF预测编码340个氨基酸,理论分子量为37.41 kDa,等电点为5.09;蛋白含有1个Inhibitor_I29和1个Peptidase_C1A结构域,与拟南芥SAG12的相似性高达61%,将基因命名为HbSAG12H1。表达分析显示,与AtSAG12在衰老叶片中的特异表达不同,在分析的各种组织中,HbSAG12H1仅在根中被检测到,表现出明显的组织特异性。本研究为下一步的功能研究与利用奠定了基础。In this study,the rubber tree （Hevea brasiliensis） was used as the material,a 1048 bp cDNA was isolated from its mot tissue with specific primers,and its sequence was analyzed.The result showed that this cDNA contained a 1023 bp long open reading frame （ORF）,including 13 bp 5 UTR and 12 bp 3＇UTR.It was predicted to encode 340 amino acids with a theoretical molecular weight （Mw） of 37.41 kDa and isolectric point （pI） of 5.09.The deduced polypeptide contained one Inhibitor_I29 domain and one Peptidase_C1A domain and exhibit high identity （61％） to Arabidopsis SAG12,thereby,this gene was designated as HbSAG12H1.Compared with the specific expression in senescent leaves ofAtSAG12,semi-quantitative RT-PCR analysis indicated that HbSAG12H1 exhibited a root-specific expression pattern in examined tissues.This result provided a basis for function research and utilization.

关 键 词：巴西橡胶树 拟南芥 SAG12基因 根特异性表达 

分 类 号：S794.1[农业科学—林木遗传育种]