鱼腥草素钠预防生物被膜引起的早期肺气虚证的研究  

Effects of Sodium houttuyfonate prevent the happening of the lung Qi deficiency syndrome in early-infected rats inducted by bacterial biofilm

作　　者：黄卫锋[1] 段强军 吴大强[1] 濮燕屏[1] 邵菁[1] 程惠娟[1] 

机构地区：[1]安徽中医药大学中西医结合临床学院,安徽合肥230038

出　　处：《时珍国医国药》2014年第11期2577-2579,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81173629)

摘　　要：目的研究鱼腥草素钠早期抗大鼠呼吸道生物被膜菌感染预防肺气虚证发生的作用。方法健康SD大鼠随机分为正常对照组、铜绿假单胞菌(Pseudomonas aeruginosa,Pa)标准株(ATCC27853)和铜绿假单胞菌临床株(AH16)分别包括模型组、鱼腥草素钠(SH)治疗组和阿奇霉素(AZM)阳性对照组。将呼吸道生物被膜菌铜绿假单胞菌标准株(Pa)和临床株(AH16)以滴鼻法感染大鼠,并辅以寒冷、疲劳刺激引起大鼠呼吸道慢性感染,诱导大鼠肺气虚证的发生。治疗组与阳性对照组于感染成功后分别用鱼腥草素钠及阿奇霉素治疗。观察各组证候表现、血气变化,酶联免疫吸附法测定肺泡灌洗液中白细胞介素-8(IL-8)的含量变化,HE染色镜下观察肺支气管组织器官病理改变。结果模型组的大鼠证状符合肺气虚证的证候表现;药物治疗组肺气虚证状不明显;血气分析表明,模型组大鼠血液中动脉氧分压(Partial pressure of oxygen,PO2)均显著下降,与正常对照组比较,P<0.05,或P<0.01,而二氧化碳分压(Partial pressure of carbon dioxide,PCO2)均显著升高与正常对照组比较(P<0.05);治疗组大鼠血液中PO2和PCO2变化不明显,与正常对照组比较P>0.05;模型组大鼠肺部灌洗液中的IL-8的含量均明显升高,与正常对照组比较,P<0.05,或P<0.01;病理学检查模型组肺及支气管炎症明显,肺部特征符合慢性支气管炎、肺气肿病理变化,鱼腥草素钠治疗组和阿奇霉素阳性大鼠肺部无病理变化出现。结论鱼腥草素钠早期干预铜绿假单胞菌生物被膜呼吸道感染的大鼠能预防肺气虚证的发生。Objective To explore the effects of Sodium houttuyfonate initially prevent the happening of the lung Qi deficiency syndrome in rats inducted by pseudomonas aeruginosa biofilm. Methods Healthy SD rats were randomly divided into normal control group,P. aeruginosa standard strains（ATCC27853,Pa） group and clinical strains of P. aeruginosa（AH16） group including the model group,Sodium houttuyfonate（SH） treatment group and azithromycin（AZM） positive control group,respectively. The cultures of standard（Pa） and clinical（AH16） strains were dropped into the noses of the rats combined with stimulations of cold and fatigue,respectively,causing respiratory tract of chronic infection in rats to induce the occurrence of lung Qi deficiency syndrome in rats. After successful infected,the treatment group and positive control group of animals were treated by sodium houttuyfonate and azithromycin,respectively. The syndrome changes in blood gas were observed. The changes of Interleukin-8（IL-8） in alveolar lavage fluid were determined using Enzyme-linked immunosorbent assay. The HE dyeing observation lung and bronchial tissue pathological variations were identified under microscope. Results The rat features in model group accorded with the symptoms of lung Qi deficiency syndrome; the symptom of the drug therapy group was not significant comparing to the lung Qi deficiency symptoms of. Blood gas analysis showed that significant change compared with normal control group in the model group rats; the infections blood arterial partial pressure of oxygen（PO2） were dropped significantly（P〈0. 05,or P〈0. 01）,while partial pressure of carbon dioxide（PCO2） were significantly higher（P〈0. 05）. Between treatment group compared with normal control group,PO2 and PCO2in rat blood were no significant difference（P〉0. 05）; Compared with normal control group,IL-8 levels of model group rats lung lavage fluid were significantly elevated（P〈0. 05 or P〈0. 01）; Pathological analysis showed that mode

关 键 词：鱼腥草素钠 铜绿假单胞菌 生物被膜 肺气虚证 

分 类 号：R285.5[医药卫生—中药学]