脱氢紫堇碱和巴马汀协同l-THP抗吗啡成瘾药效初探  

Primary Study on Synergistic Efficacy of Dehydrocorydaline and Palmatine with l-THP in the Treatment of anti-morphine Addiction

作　　者：涂平[1] 白威峰[1] 余守洋[1] 邱成楷 吴明松[1] 罗素元[1] 

机构地区：[1]遵义医学院,贵州遵义563099

出　　处：《时珍国医国药》2014年第11期2587-2589,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860373);贵州省科技厅社会攻关项目(黔科合SY字[2013])3065

摘　　要：目的初步探寻延胡索中1-2种与左旋延胡索乙素（l-THP）协同抗吗啡成瘾的有效成分。方法小鼠吗啡50 mg·kg^-1腹腔1次注射,至3 h纳洛酮注射建立小鼠吗啡依赖纳洛酮催促戒断跳跃反应模型。按l-THP、脱氢紫堇碱和巴马汀各自在延胡索中自然存在比例（l-THP∶脱氢紫堇碱：巴马汀=1∶2.31∶0.46）配置成＂l-THP-脱氢紫堇碱-巴马汀＂混合液,分别观察l-THP 3 mg·kg^-1单体、混合液（混合液内所含l-THP的量等同l-THP单体治疗组的剂量）灌胃治疗后,对模型小鼠戒断10 min内跳跃反应发生率和每鼠跳跃次数,以及1 h体重改变的影响。结果模型组较生理盐水对照组小鼠跳跃次数和跳跃反应百分率明显增加（P〈0.01）,体重下降（P〈0.01）;l-THP 3 mg·kg^-1组与模型组各指标数据接近（P〉0.05）;混合液组跳跃次数和跳跃反应百分率比模型组均显著减少（P〈0.05）,而体重下降幅度减轻（P〈0.05）。结论从行为学方面初步表明,延胡索水提液中的脱氢紫堇碱和巴马汀成分具有协同l-THP抗吗啡成瘾的药效作用。Objective To study 1-2 components within Corydalis yanhusuo synergy with l-THP in the Treatment of anti-morphine addiction. Methods The Naloxone-induced withdrawal jumps in the morphine-dependent experimental mice model was established by injecting morphine in mice with the dose of 50 mg·kg^-1and naloxone after 3 h. The l-THP,dehydrocorydaline and palmatine were mixed in an appropriate ratio based on the natural part of each component in corydalis yanhusuo（l-THP：dehydrocorydaline：palmatine = 1：2. 31：0. 46） to compare the effect of the mixed-drug with that of l-THP（3 mg·kg^-1） on withdrawal symptoms and weight loss in the model mice. Results Compared with the physiological saline treatment group,morphine-dependent experimental mice group showed a remarkably increase in jumping times and jumping rate and weight loss（P〈0. 01,P〈0. 05,P〈0. 05）. l-THP treatment group showed the same outcomes as morphine-dependent experimental mice group. Compared with the morphine-dependent experimental mice group,mixed-drug treatment group showed a remarkably decrease in jumping times and jumping rate and weightloss（P〈0. 01,P〈0. 05,P〈0. 05）. Conclusion These results showed that there was synergistic efficacy of dehydrocorydaline and palmatine with l-THP in the Treatment of anti-morphine Addiction.

关 键 词：吗啡成瘾 左旋延胡索乙素 脱氢紫堇碱 巴马汀 协同作用 

分 类 号：R285.5[医药卫生—中药学]