基于单链核酸-纳米金-汞模拟酶的汞离子检测方法研究  

Study on Detection of Hg(Ⅱ) Based on Single Nucleic Acid/Au NPs/Mercury Ion Enzyme Mimetics

作　　者：彭池方[1] 泮秋立 谢正军[1] 万芳梅 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《分析测试学报》2014年第11期1312-1316,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(31371767);十二五国家科技支撑计划项目(2012BAK08B01)

摘　　要：将汞离子（Hg2＋）沉积到吸附单链核酸（ssDNA）的纳米金（AuNPs）表面后,可以提高纳米金的模拟过氧化物酶活性,基于此原理可实现Hg2＋的高灵敏检测。研究发现ss DNA能够促进Au NPs-Hg2＋的类似过氧化物酶活性,且随着ss DNA浓度的增加,该作用呈增强趋势。在优化反应条件下,将ss DNA-Au NPs-Hg2＋模拟过氧化物酶用于Hg2＋的检测,Hg2＋的检测线性范围为10-1 000 nmol/L,检出限可达3.0 nmol/L。该检测方法具有简便快速、成本低、稳定性高等优点,有望用于环境、食品等样品中Hg2＋的检测。Mercury（ Hg2＋） ions deposited on the surface of gold nanoparticles （AuNPs） absorbing single strand DNA （ssDNA） can promote the peroxidase-like activity of AuNPs. Based on this, a highly sensitive detection of Hg2＋ was developed. Here it was found that ssDNA can promote the ac- tivity of AuNPs- Hg2＋ mimetic peroxidase due to the adsorption of ssDNA on the AuNPs surface. The catalytic activity of AuNPs - Hg2＋ mimetic peroxidase increases with increase of ssDNA concen- tration. The ssDNA - AuNPs - Hg2＋ mimetic peroxidase can be applied in the detection of Hg2＋ in a linear range of 10 - 1 000 nmol/L with a detection limit as low as 3.0 nmol/L under optimized condi- tion. The method was simple, rapid, low cost and stable, and had high application potential to de-tect Hg2＋ in the environment, food and other samples.

关 键 词：Hg2+ 检测 纳米金 过氧化物模拟酶 

分 类 号：O657.3[理学—分析化学] O614.243[理学—化学]