用HPLC测定羟基苯乙酸-N,N-二甲氨基甲酰甲酯含量的研究  

Research on the determination methods of N,N-dimethylcarbamoylmethyl-p-hydroxyphenylacetate

作　　者：李宗霖[1] 麻纪斌 曲秦 翟帆[1] 舒泉湧 秦湫红 

机构地区：[1]陕西新药技术开发中心,陕西西安710075

出　　处：《应用化工》2014年第10期1922-1924,共3页Applied Chemical Industry

摘　　要：以高效液相色谱法（HPLC）测定对羟基苯乙酸-N，N-二甲氨基甲酰甲酯的含量，采用Thermo Syneronis C18柱（250mm×4.6mm，5μm），以乙腈-水=30：70（V/V）为流动相，流速1.0mL/min，检测波长275nm。结果表明，对羟基苯乙酸-N，N-二甲氨基甲酰甲酯在0.1-1.0μg范围内线性关系良好，加样回收率为98.8%，RSD=1.08%。该方法准确，便捷，重现性好，适用于对羟基苯乙酸-N，N-二甲氨基甲酰甲酯的含量测定及工业生产中的质量控制。Determination of N,N-dimethylearbamoylmethyl-p-hydroxyphenylaeetate by HPLC. The analyti-eal eolumn was Thermo Syneronis C18（250 mm ×4. 6 mm,5μm）. The mobile phase eonsisted of aeetoni-trile-water（30：70）. The flow rate was 1. 0 mL/min and the deteetion wavelength was 275 nm. Results showed that the linear range of N,N-dimethylearbamoylmethyl-p-hydroxyphenylaeetate was at 0. 1 -1. 0 μg,and the average reeovery was 98. 8%,RSD was 1. 08%. The method is aeeurate,eonvenient, good reprodueibility and ean be used for the eontent determination of N,N-dimethylearbamoylmethyl-p-hydroxyphenylaeetate. Also suitable for the quality eontrolling during the industrial produeing process.

关 键 词：对羟基苯乙酸-N N-二甲氨基甲酰甲酯 高效液相色谱法 含量测定 

分 类 号：TQ063[化学工程] R927.2[医药卫生—药学]