用于细胞排列的介电泳微流控芯片制备与实验研究  

Preparation and Experimental Study on Dielectrophoresis Based Microfluidic Chip for Cell Patterning

作　　者：张洋[1] 张晓飞[1] 白国花[2] 方明[2] 谭秋林[1,2] 熊继军[1,2] 孙东[1,2,3] 

机构地区：[1]中北大学电子测试技术国家重点实验室,太原030051 [2]仪器科学与动态测试教育部重点实验室,太原030051 [3]香港城市大学机械及生物医学工程系,香港九龙999077

出　　处：《分析化学》2014年第11期1568-1573,共6页Chinese Journal of Analytical Chemistry

基　　金：supported by the National Natural Science Foundation of China(Nos.51205373,51275491)

摘　　要：设计并制作了一种应用于细胞排列的介电泳微流控芯片,以实现细胞的非接触、批量排列。芯片主要包括PDMS微通道和“台阶”形ITO微电极。运用仿真软件COMSOL分析了微电极所形成的电场分布,确定了最大电场强度的位置；利用MEMS加工工艺制备了ITO微电极和PDMS微通道,PDMS微通道与带有ITO电极的载玻片经过氧等离子表面处理后,对准键合获得最终的微流控芯片。通过不同频率下的介电泳实验,实现了酵母菌细胞的介电泳运动,并确定了正、负介电泳运动的电场频率。结果表明,酵母菌细胞在溶液电导率为60μS/cm的环境下,1-10kHz时,发生负介电泳运动；0.5-10MHz时,发生正介电泳运动；50kHz时,没有发生介电泳运动。并在施加8Vp-p,5MHz交流电压信号的条件下,实现了酵母菌细胞沿“台阶”形电极边缘直线排列。A dielectrophoresis-based microfluidic chip applied to cell spatterning is designed and fabricated, and it demonstrates non-contact and batch manipulation of cells. The microfluidic chip employs a PDMS microchannel and two ITO electrodes, which are designed as a＂step＂ shape. The distribution of electric field caused by the microelectrodes is simulated by finite element simulation software, COMSOL. The position of the maximum intensity of electric field is also determined. The ITO microelectrodes and the PDMS microchannel are fabricated using MEMS fabrication process. After oxygen plasma surface treatment, the PDMS microchannel and glass substrate with the ITO microelectrodes are aligned and bonded to form experimental microfluidic chip. Through DEP experiment with the varying frequencies, DEP response of yeast cells is examined, and the electric field frequency of the both positive and negative DEP responses are confirmed. The results showed that yeast cells in solution conductivity of 60 μS/cm had negative DEP movement at the frequency of 1 kHz to 10 kHz, positive DEP movement at the 500 kHz to 10 MHz, and no DEP movement at the 50 kHz. Under the condition of the sinusoidal potential of 8Vp-p and the electric field frequency of 5 MHz, the yeast cells were aligned into chains along the ＂step＂ edge of microelectrodes.

关 键 词：介电泳 微流控芯片 有限元仿真 细胞排列 

分 类 号：O658.9[理学—分析化学]