二氧化硅室温磷光纳米材料的制备及其细胞成像应用  

Fabrication and Cell Imaging of Room Temperature Phosphorescent Silica Nanomaterials

作　　者：张曼曼[1] 王立强[1] 肖丹[2] 庄贞静[1] 

机构地区：[1]华侨大学分子药物学研究院,泉州362021 [2]四川大学化学学院,成都610064

出　　处：《分析化学》2014年第11期1586-1591,共6页Chinese Journal of Analytical Chemistry

基　　金：福建省自然科学基金(No.2010J05026)资助项目~~

摘　　要：利用溶胶-凝胶法制备了非金属掺杂的二氧化硅室温磷光纳米材料,探讨了这种纳米材料的可控制备、光谱性质、细胞毒性和细胞成像。通过TEM和XRD对此材料的形貌和结构进行表征。结果表明,所制备材料为二氧化硅非晶材料,粒径约为50 nm。通过荧光分光光度计对此材料的荧光和磷光光谱进行表征,结果表明,此材料最大激发峰处于280 nm,最大荧光发射峰处于335 nm,最大磷光发射峰处于440 nm。此材料还具有良好的环境稳定性,在空气中放置3个月,其磷光强度基本不发生变化,表明其具有良好的长期稳定性。MTT及激光共聚焦显微成像分析结果表明,此材料具有良好的生物相容性且可顺利进入细胞,并定位在溶酶体,因此有望作为新型溶酶体纳米探针在生物医学领域得以广泛应用。A phosphor based on silica nanoparticles was prepared using a sol-gel method.The controllable synthesis, spectroscopic properties, cytotoxicity and cell imaging of these nanomatericals were examined by using photoluminescence spectra, TEM, XRD, confocal microscopy and other characterized measurements. The results demonstrated that the obtained sample was silica with diameter about 50 nm. The maximum fluorescence excitation and emission wavelengths of the silica nanomatericals were 280 nm and 335 nm, and the maximum phosphorescence excitation and emission wavelengths of the silica nanomatericals were 280 nm and 440 nm. The obtained silica sample possessed room temperature phosphorescence that was stable against environmental changes. The obtained sample was stored in air at ambient conditions and its phosphorescence remained unchanged after 3 month demonstrated its long-term stability. The result of methyl thiazolyl tetrazolium （ MTT） and cell imaging experiments suggested that the synthesized silica nanoparticles were feeble cytotoxicity and could be uptaken by cells at the lysosomal compartment. Therefore these nanoparticles could serve as bioprobes for cell imaging.

关 键 词：二氧化硅 溶胶-凝胶法 室温磷光 细胞毒性 细胞成像 

分 类 号：TB383.1[一般工业技术—材料科学与工程]