表面电性可控的磁性微球的制备、表征及其在基因组DNA萃取中的应用  

Preparation,Characterization and Application for Genomic DNA Extraction of a Magnetic Microsphere with Surface Charge Switching

作　　者：黄华斌[1] 庄峙厦[2] 杨朝勇[2] 王小如[1] 

机构地区：[1]厦门华厦职业学院检验与科学技术系,厦门361024 [2]厦门大学化学化工学院,厦门361005

出　　处：《分析化学》2014年第11期1598-1603,共6页Chinese Journal of Analytical Chemistry

基　　金：厦门市科技计划基金(No.3502720123046)资助

摘　　要：运用水热法、Stber法制备了具有核壳型结构的超顺磁性Fe3O4@SiO2微球,运用氨丙基三乙氧基硅烷(APTES)对Fe3O4@SiO2微球表面进行修饰,得到表面电性可控的氨基化Fe3O4@SiO2磁性微球,运用透射、扫描电镜表征微球形貌,采用Zeta电位研究微球表面的荷电特性。将氨基化Fe3O4@SiO2微球用于人类全血中基因组脱氧核糖核酸(DNA)的萃取,开发了固相萃取方法,并探究微球与DNA的作用机理,对提取产物进行凝胶电泳和PCR实验。结果表明,自制的微球成功地从全血中提取出纯度较高的基因组DNA,提取率约70%,微球对基因组DNA的饱和吸附量约为40 ng/μg,提取液可直接用于进一步的生物分析。Super paramagnetic SiO2 @ Fe3O4 microsphere with core-shell structure was prepared by the method of hydro-thermal and Stober method, and modified by （3-aminopropyl） triethoxysilane （ APTES） . The obtained amino-Fe3 O4@SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3 O4@SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3 O4@SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/μg, and the elution could be directly used for further bio-analysis.

关 键 词：表面电性可控 磁性微球 DNA萃取 

分 类 号：O629.74[理学—有机化学] O658.2[理学—化学]