RP-HPLC法测定QOA的含量及有关物质  

RP-HPLC determination of content and related substances of QOA

作　　者：杜云[1] 戴韵卿 顾维[3] 李建新[1] 

机构地区：[1]南京大学化学化工学院,南京210093 [2]江苏大学,镇江212013 [3]南京市妇幼保健院,南京210004

出　　处：《分析试验室》2014年第11期1332-1334,共3页Chinese Journal of Analysis Laboratory

摘　　要：建立了测定QOA含量及有关物质的RP-HPLC方法。采用Sepax C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈与体积分数0.1%乙酸水溶液（体积比为10：90）,流速1.0 m L/min,检测波长241 nm,进样体积为20μL。QOA质量色谱峰与其相邻杂质峰能完全分离,QOA质量浓度在0.5-50μg/m L范围内呈良好的线性关系（A=48387ρ-736.4,γ=0.9999）,检出限为10 ng/m L（S/N≥3）。方法 RSD为0.45%,重复性试验RSD为0.37%,稳定性试验RSD为0.21%。To establish RP-HPLC method for the determination of QOA and its related substances. Sepax C18 （250 mm ×4. 6 mm,5 μm） column was used. The flow rate was 1.0 mL/min. The detection wavelength was 241 nm. The mobile phase consisted of 0. 1% acetic acid-acetonitrile （ 90 ： 10） at the flow rate of 1.0 mL/min. Injection volume was 20 μL. QOA was separated completely from impurities. The linear range of QOA was 0. 5 - 50 μg/mL（A = 48387p - 736. 4, γ = 0. 9999 ） ; the minimum detection limit was 10ng （ S/N≥ 3 ）. The content RSDs of the precision, the reproducibility and the stability test were 0. 45%, 0. 37% ad 0. 21%.

关 键 词：高效液相色谱 QOA 纯度测定 杂质分析 

分 类 号：O657.7[理学—分析化学]