松生等总黄酮提取工艺及镇痛抗炎作用研究  

Analgesia and Anti-inflammatory Effect of Total Flavonoids Extracted from Euphorbia prolifera

作　　者：金岩[1] 黄山[1] 赵磊[2] 裴小娜[1] 

机构地区：[1]青岛科技大学化工学院药学系,青岛266042 [2]甘肃中医学院甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000

出　　处：《医药导报》2014年第11期1411-1415,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(81360686);甘肃省教育厅硕士生导师项目(1106-02)

摘　　要：目的研究松生等总黄酮的最佳优化提取工艺,并对不同极性溶剂提取部分的镇痛抗炎作用进行研究。方法通过单因素实验和正交实验,考察提取时间、料液比、乙醇浓度和提取次数对松生等总黄酮提取率的影响;采用小鼠冰醋酸扭体法及二甲苯致小鼠耳肿胀模型筛选松生等不同溶剂提取部分镇痛抗炎作用。结果回流提取法提取松生等总黄酮的最佳条件为:提取时间3 h、料液比1∶30、乙醇浓度60%、提取2次,黄酮提取率最高5.63%;松生等乙醇提取物、正丁醇提取物和乙酸乙酯提取物均能够抑制冰醋酸引起的小鼠扭体反应和二甲苯引起的小鼠耳肿胀。结论该工艺提取松生等总黄酮提取率高,方法简便,重复性好。松生等乙醇提取物、正丁醇提取物和乙酸乙酯提取物均具有明显镇痛抗炎作用。Objective The study aimed to investigate the best extraction process and the analgesia and antiinflammatory properties of total flavonoids from Euphorbia prolifera. Methods Through single factor experiment and orthogonal test,factors that affect the extraction yield of total flavonoids were studied,including the extraction time,solid to liquid ratio,ethanol concentration and extraction times. The mice models of ear edema induced by xylene and twisting induced by acetic acid were used to evaluate the analgesia and anti-inflammatory effects of different extractions from Euphorbia prolifera. Results Total flavonoids were extracted by the refluxing method,and the optimum conditions were extracting for 3 hours,solid to liquid ratio of 1∶ 30,ethanol content of 60% in the solvent,and processed for 2 times. The highest extraction yield of total flavonoids from Euphorbia prolifera was 5. 63%. The ethanol,ethyl acetate and n-butanol extracts decreased twisting and ear swelling in mice. Conclusion The extraction for total flavonoids from Euphorbia prolifera is simple,efficient and reproducible. The ethanol,ethyl acetate and n-butanol extracts of total flavonoids have obvious analgesia and anti-inflammatory effects.

关 键 词：松生等 总黄酮 回流提取 镇痛 抗炎 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]