bFGF对成纤维细胞增殖和胶原及纤维连接蛋白的影响  

Effects of Basic Fibroblast Growth Factor on Proliferation as well as Collagen and Fibronectin Expression on Fibroblasts

作　　者：武艳[1] 杨岚[2] 张羽飞[1] 袁晓环[1] 初彦辉[1] 

机构地区：[1]牡丹江医学院医药研究中心,牡丹江157011 [2]牡丹江医学院红旗医院眼科,牡丹江157011

出　　处：《医药导报》2014年第11期1416-1419,共4页Herald of Medicine

基　　金：2014年黑龙江省教育厅青年学术骨干支持计划项目(1254G064)

摘　　要：目的探讨碱性成纤维细胞因子(b FGF)调节皮肤创伤修复的作用机制。方法采用组织块贴壁法培养来源于正常皮肤和增生性瘢痕的成纤维细胞(FB),加入含有不同浓度b FGF(0,0.1,1,10,100,1 000 ng·m L-1)的无血清培养液培养72 h,采用细胞计数法和锥虫蓝染色检测各组FB增殖和凋亡,酶联免疫吸附法(ELISA)和逆转录PCR(RT-PCR)分别检测Ⅰ型、Ⅲ型胶原和纤维连接蛋白浓度及基因表达情况。结果 b FGF促进FB增殖,当浓度过高时则抑制FB增殖,促进其凋亡,并且增生性瘢痕FB增殖较来源于正常皮肤的FB缓慢;b FGF明显抑制增生性瘢痕FBⅠ型胶原产生,对来源于正常皮肤FB无影响;b FGF上调来源于正常皮肤FB的纤维连接蛋白基因表达,对增生性瘢痕FB中的表达无影响;b FGF对两种来源的FBⅢ型胶原的分泌和表达均无显著作用。结论 b FGF对来源正常皮肤和增生性瘢痕的FB具有不同的影响和作用机制,可能对创伤修复早期和瘢痕形成过程发挥作用。Objective To explore the mechanism and effects of basic fibroblast growth factor（ b FGF） on skin wound healing. Methods Fibroblasts（ FB） were isolated from normal skin and hypertrophic scar and cultivated by direct adherence method. FB were then treated with different concentrations of b FGF（ 0,0. 1,1,10,100,1 000 ng·m L^-1） and cultivated with serum-free medium for 72 hours. The proliferation and apoptosis of FB in each group were detected by cell counting and trypan blue staining. Content and gene expression of type Ⅰ and type Ⅲ collagen and fibronectin were determined by ELISA and RTPCR,respectively. Results b FGF promoted the proliferation of FB at low concentrations promoted apoptosis of FB at higher concentrations. The proliferation of FB from hypertrophic scar was slower than that from the normal skin. b FGF significantly inhibited type Ⅰ collagen production from hypertrophic scar FB but not from the normal skin. Moreover,b FGF up-regulated fibronectin expression in the normal fibroblasts,but not in the hypertrophic scar. No change in type Ⅲ collagen expression and production was observed in FB from either source. Conclusion b FGF has differential effects and mechanisms on FB of the normal skin and hypertrophic scar,suggesting that b FGF may play a role in early phase of skin wound healing and scar formation.

关 键 词：碱性成纤维细胞因子 成纤维细胞 增生性瘢痕 创伤修复 

分 类 号：R982[医药卫生—药品] R965[医药卫生—药学]