艾叶发酵物治疗系统性白念珠菌感染  

Therapeutic Effects of Artemisia Argyi Ferment Substance on Systemic Candida Albicans Infection

作　　者：白静[1] 胡雷[1] 张丽 田春雨[1] 庞得全[1] 薄海美[1] 韩淑英[1] 

机构地区：[1]河北联合大学基础医学院药理系,唐山063000 [2]河北省唐山市工人医院麻醉科,唐山063000

出　　处：《医药导报》2014年第11期1438-1441,共4页Herald of Medicine

基　　金：唐山市科技计划项目(12130208A-2)

摘　　要：目的观察艾叶发酵物对系统性白念珠菌感染的治疗作用,并探讨其可能机制。方法建立免疫抑制小鼠系统性白念珠菌感染模型。将模型小鼠随机分为模型对照组,艾叶发酵物小、中、大剂量组（100,200,400 mg·kg^-1）及氟康唑组（20 mg·kg^-1）,每组30只。小鼠灌胃给予相应治疗药物,bid,连续给药5 d。计算模型建立后21 d各组小鼠生存率,酶联免疫吸附（ELISA）法测定小鼠血清γ-干扰素（IFN-γ）、白细胞介素2（IL-2）水平,噻唑蓝（MTT）法检测小鼠脾脏T淋巴细胞增殖活性,平板计数法测定小鼠肝、肾组织活菌数目。结果与模型对照组比较,艾叶发酵物中、大剂量组和氟康唑组小鼠存活率,血清IFN-γ、IL-2水平及脾脏T淋巴细胞增殖活性均较高,而肝、肾组织白念珠菌数目较低（P〈0.01）;与艾叶发酵物小剂量组比较,中、大剂量组和氟康唑组上述各指标变化更显著（P〈0.05或P〈0.01）,但中、大剂量组与氟康唑组间比较,各指标差异无统计学意义（P〉0.05）。结论艾叶发酵物200-400 mg·kg^-1对系统性白念珠菌感染有一定治疗作用,其机制可能与其提高T淋巴细胞增殖活性及IFN-γ、IL-2水平相关。Objective To investigate the therapeutic effects and mechanism of Artemisia argyi ferment substance on systemic Candida albicans infection. Methods The model of systemic Candida albicans infection was established in immunosuppressed mice. The model mice were randomly divided into the model control,Artemisia argyi ferment substance（ AAFS）at different doses（ 100,200,and 400 mg·kg^- 1） and fluconazole group（ 20 mg·kg^- 1）,30 mice in each. Mice in each treatment group were given therapeutic drugs by gavage for 5 consecutive days,twice daily. The survival of mice was determined 21 days after the model was set up. The serum levels of IFN-γ and IL-2 were determined by ELISA. The proliferation activity of T lymphocyte in the spleen was detected by MTT assay. The number of living fungi in liver and kidney tissues was counted. Results Compared with the model control,AAFS at middle and high doses and fluconazole significantly increased the survival rate of mice,the serum levels of IFN-γ and IL-2,and the proliferation activity of T lymphocyte in the spleen,but decreased the number of living fungi in tissues（ P〈 0. 01）. Compared with low dose AAFS,middle and high doses of AAFS and fluconazole showed significantly different effect on each index（ P〈 0. 05 or P〈 0. 01）,but there was no difference among these groups（ P〉 0. 05）. Conclusion AAFS at200- 400 mg·kg- 1has inhibitory effects on systemic Candida albicans infection in mice,the mechanism of which is related to increasing the proliferation of T lymphocyte in spleen and the levels of IFN-γ and IL-2 in serum.

关 键 词：艾叶发酵物 感染白念珠菌 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]