高效液相色谱-蒸发光散射检测器法测定人参健脾丸中黄芪甲苷含量  

Determination of Astragloside Ⅳ Content in Renshen Jianpi Pill by HPLC- ELSD

作　　者：陈健 胡幸 吕紫璇 张传平 

机构地区：[1]重庆市涪陵食品药品检验所,重庆408000

出　　处：《中国药业》2014年第22期55-56,共2页China Pharmaceuticals

摘　　要：目的建立测定人参健脾丸中黄芪甲苷含量的方法。方法色谱柱为Agile Eclipse XDB C18（250mm×4．6mm，5μm），以甲醇-水（75：25）为流动相，流速1．0mL／min，柱温为30℃；ELSD检测条件，漂移管温度40℃，增益40结果黄芪甲苷进样量在1．465—7．325μg范围内与峰面积呈良好线性关系（r=0．9995），加样回收率为98．48％，RSD为0．64％（n=6）。结论所建立的方法简单、准确、重复性好，可用于人参健脾丸的质量控制。Objective To establish a HPLC-ELSD method for the determination of the astragaloside IV content in Renshen Jianpi Pill. Methods The Agilent Eclipse XDB Ct8 column（250 mm×4.6 mm,5 μm） was used with methanol-water（75：25） as the mobile phase;the flow rate was 1.0 mL/min;the column temperature was 30 ℃. The ELSD conditions were the drift tube temperature of 40 ℃ and the gain value of 4. Results The sample size of astragaloside IV in the range of 1. 465- 7. 325 μg showed the good linear rela- tion with the peak area（r=0. 999 5）,the average recovery rate was 98.48%, RSD was 0. 64% （n=6）. Conclusion The established method is simple and accurate with good reproducibility,which can be used to control the quality of Renshen Jianpi Pill.

关 键 词：高效液相色谱-蒸发光散射检测器法 人参健脾丸 黄芪甲苷 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]