HPLC法同时测定白花蛇舌草中对香豆酸和熊果酸的含量  

Determination of p-Coumaric Acid and Ursolic Acid in Hedyotis Diffuse by HPLC

作　　者：黄祝刚 于亮[1] 左琳[1] 满其倩 容蓉[1] 巩丽丽[1] 蒋海强[1] 吕青涛[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《化学分析计量》2014年第6期4-7,共4页Chemical Analysis And Meterage

基　　金：国家自然科学基金项目(81173614)

摘　　要：建立高效液相色谱法同时测定白花蛇舌草药材中对香豆酸和熊果酸含量的方法。采用EclipseSB—C18色谱柱（250mm×4．6mm，5um），以甲醇-0．05％甲酸梯度洗脱，流量1mL／min，对香豆酸和熊果酸的检测波长分别为310nm和210nm，柱温30℃。对香豆酸和熊果酸含量分别在0．00179-0．02864mg／mL和0．0213～0．3408mg／mL范围内与色谱峰面积呈良好的线性关系，线性相关系数，分别为0．9998，0．9996。对香豆酸和熊果酸的平均加标回收率分别为98．59％，98．23％，测定结果的相对标准偏差分别为0．81％，0．94％（n=6）。样品溶液在24h内稳定。该方法适用于白花蛇舌草药材中对香豆酸和熊果酸的含量测定。A method for simultaneously determination ofp-coumaric acid and ursolic acid in hedyotis diffusa was established. An Eclipse SB-C18 column （250 mm × 4.6 mm, 5 μm） was adopted with a mobile phase of methanol-0.05% formic acid at the flow rate of 1.0 mL/min. The detection wavelength was set at 310 nm and 210 nm for p-coumaric acid, ursolic acid, respectively, and the column temperature was 30℃. The calibration curves were linear in the ranges of 0.001 79-0.028 64 mg/mL（r=0.999 8） for p-coumaric acid and 0.021 3-0.340 8 mg/mL（r=0.999 6） for ursolic acid. The average recoveries of p-coumaric acid and ursolic acid were 98.59% and 98.23%, respectively, and the detection limit of p-coumaric and ursolic acid was 0.81%, 0.94%（n=6）, respectively. The sample solution was steady within 24 h. The method is suitable for determination ofp-coumaric acid and ursolic acid in hedyotis diffusa.

关 键 词：白花蛇舌草 对香豆酸 熊果酸 HPLC 

分 类 号：O657.7[理学—分析化学]