盐酸吡哆辛在聚烟酸修饰电极上的电化学行为及测定  

Electrochemical Behavior and Determination of Pyridoxine Hydrochloride at the Poly Nicotinic Acid Modifi ed Electrode

作　　者：顾小燕[1] 唐鹏鹏[1] 李宁波[1] 刘欢[1] 俞静[1] 何凤云[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,南京211171

出　　处：《化学分析计量》2014年第6期15-19,共5页Chemical Analysis And Meterage

基　　金：教育部重点实验室开放研究基金项目(KLACLS08001);江苏省大学生创新训练项目(2013053)

摘　　要：采用电聚合方法制备了聚烟酸修饰玻碳电极（PNA／GCE），并利用循环伏安法（CV）和差分脉冲伏安法（DPV）考察了盐酸吡哆辛（VB6）在该修饰电极上的电化学行为。结果表明，VB6在PNA／GCE上的氧化峰电流显著提高，电极反应为扩散控制的一电子两质子反应。利用差分脉冲伏安法对VB6进行测定，线性范围为0．08-400μmol／L，VB6的检出限为0．02μmol／L，测定结果的相对标准偏差为3．1％（”=8），加标回收率为95．8％-103．7％。该方法可用于VB6片中VB6含量的测定。Poly（nicotinic acid） modified electrode （PNA / GCE） was prepared and the electrochemical behavior of pyridoxine hydrochloride（VB6） was investigated at the modified electrode by cyclic voltammetry（CV） and differential pulse voltammetry（DPV）. Results showed that the oxidation cux-rent of VB6 at the PNA / GCE was obviously larger than that at GCE. The oxidation process of VB6 was an irreversible diffusion-controlled process involving one electron and two protons. The liner range between the peak current of DPV and the concentration of VB6 was 0.08-400 μmol/L, and the detection limit was 0.02 μmol/L. The modified electrode showed a good reproducibility with a relative standard deviation of 3.1%（n=8）. The recoveries were between 95.8% and 103.7%. The method can be applied to the analysis of vitamin B6 content in vitamin B6 tablets.

关 键 词：聚烟酸 盐酸吡哆辛 循环伏安法 差分脉冲伏安法 

分 类 号：O657.1[理学—分析化学]