慢性尿道炎患者细菌L型及支原体检测分析  

Detection and analysis of L-form bacteria and mycoplasma from patients with chronic urethritis

作　　者：顾丽芳 吕火祥[2] 朱慧 胡守利 

机构地区：[1]余杭区第二人民医院,浙江杭州311121 [2]浙江省人民医院,浙江杭州310014

出　　处：《中国卫生检验杂志》2014年第22期3273-3274,3278,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的了解本地区慢性尿道炎患者细菌L型及支原体分离和耐药性情况,为临床合理用药提供可靠依据。方法采用细菌L型增菌和分离培养法及液体培养法对1 425例慢性尿道炎患者的中段尿进行细菌L型及支原体的培养、鉴定和药物敏感试验。结果细菌L型阳性96例,阳性率6.7%;支原体阳性528例,阳性率为37.1%,解脲脲原体(Uu)、人型支原体(Mh)以及Uu+Mh混合感染的所占比例分别为74.6%(394/528)、3.6%(19/528)和21.8%(115/528),男女性感染率分别是19.1%、38.9%,差异有统计学意义(χ2=19.81,P<0.005);药敏试验结果显示强力霉素、美满霉素、交沙霉素对所有支原体有较高的抗菌活性,喹诺酮类药物最低。结论本地区慢性尿道炎患者中支原体分离率较高,以Uu为主,且女性高于男性,对支原体体外药敏提示:强力霉素、美满霉素、交沙霉素敏感性最高。细菌L型的感染也不容忽视。Objective To investigate the isolation and drug resistance of L-form bacteria and mycoplasma from the patients with chronic urethritis in our region,so as to provide a reliable basis for clinical rational drug use.Methods The culture,identification and drug susceptibility test of L-form bacteria and mycoplasma in 1 425 cases of mid-stream urine from patients with chronic urethritis were performed by the method of enrichment separation and culture as well as the liquid culture method to L-form bacteria.Results The positive of L-form bacteria was 96 cases,the positive rate was 6.7%.528 cases of mycoplasma were positive,mixed infection of the positive rate was 37.1%,the Ureaplasma urealyticum（Uu）,Mycoplasma hominis（Mh）,and Uu ＋ Mh proportion was 74.6%（394/528）,3.6%（19/528） and 21.8%（115/528）,respectively,infection rates were 19.1% and 38.9% for men and women respectively,and the difference was statistically significant（χ2= 19.81,P 0.005）;drug susceptibility test results showed that the higher antimicrobial activity to this mycoplasma were doxycycline,minocycline,azithromycin and josamycin,and the lowest was quinolones.Conclusion In the patients with chronic urethritis in the region,mycoplasma separation rate was higher,giving priority to Uu,on women than on men,the highest drug susceptibility to mycoplasma in vitro were minocycline,doxycycline,azithromycin and josamycin.The L-form bacterial infection also don＇t allow to be ignored in these patients.

关 键 词：慢性尿道炎 支原体感染 细菌L型 药敏试验 

分 类 号：R446.5[医药卫生—诊断学]