荧光定量PCR检测新疆地区不孕不育患者UU、CT结果分析  

The detection and analysis on infections of Ureaplasma urealyticum(UU)and Chlamydia trachomatis(CT) in infertile patients of Xinjiang by Fluorescence Quantitative PCR

作　　者：杨艳 代文成 刘璇 张金花 马光娟 刘宁 

机构地区：[1]新疆乌鲁木齐妇幼保健院遗传中心,乌鲁木齐830000

出　　处：《中国优生与遗传杂志》2014年第11期143-144,50,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的利用实时荧光定量PCR技术了解新疆地区不孕不育患者解脲支原体(UU)和沙眼衣原体(CT)感染情况,通过对实验结果的统计学处理分析不孕不育与UU、CT感染率及感染量之间的关系,方法采用实时荧光定量PCR技术检测新疆地区不孕不育患者及能正常生育人群解脲支原体-DNA和沙眼衣原体-DNA含量并进行统计学分析。结果不孕不育患者UU-DNA阳性率52.18%,CT-DNA阳性率3.65%,正常对照组则分别为46.08%、1.00%,两组间的差异显著性有统计学上的意义,不孕不育组UU和CT的感染率明显高于对照组(P<0.05)。不同性别,不用民族的UU阳性检出率不同。结论新疆地区不孕不育患者中UU和CT感染是引起男女不孕不育的重要原因之一,且女性UU阳性率高于男性,维吾尔族UU阳性率高于其它民族。Objective：To detect the infection of Ureaplasma urealyticum（UU）and Chlamydia trachomatis（CT）in infertility of Xinjiang by Using Fluorescence Quantitative PCR technology,and anslyse the relationship between the infection rate of UU CT and infertility by statistical analysis. Methods：The quantities of UU and CT were detected by using Fluorescence Quantitative PCR technology in infertility and negative control group of Xinjiang,the statistics were analyzed by statistical analysis. Results：The positive rates of UU-DNA and CT-DNA were 52.18% 3.65% respectively,and the negative control were 46.08% 1.00% respectively,the differences were significant（P〈0.05）. Infection rate of UU and CT were higher in infertility patients than in the negative control group. It has different infection rate in different gender or different nations. Conclusion：The infection of UU and CT is one of the important reasons in infertility in Xinjiang,and the positive rate of UU is higher in women than in men,and Uigurs is consider to be infected by UU much easier.

关 键 词：实时荧光定量PCR 不孕不育 解脲支原体 沙眼衣原体 

分 类 号：R440[医药卫生—诊断学]