大熊猫和北极熊基因组微卫星分布特征比较分析  

Comparative Analysis of Microsatellite Sequences Distribution in the Genome of Giant Panda and Polar Bear

作　　者：李午佼[1] 李玉芝[1,2] 杜联明[1] 黄杰[1] 沈咏梅 张修月[1] 岳碧松[1] 

机构地区：[1]四川省濒危野生动物保护生物学重点实验室四川大学生命科学学院,成都610064 [2]成都中医药大学药学院,成都610075 [3]四川好医生攀西药业有限责任公司,四川西昌615000

出　　处：《四川动物》2014年第6期874-878,共5页Sichuan Journal of Zoology

摘　　要：本研究比较分析了大熊猫和北极熊全基因组序列中的1~6碱基重复的完美型微卫星序列的分布特征,通过微卫星序列搜索和统计软件MSDB分析分别得到855 018和936 238个微卫星序列,其长度总和分别是14 919 240 bp和18 434 348 bp;分别占基因组大小的0.64%和0.79%,大熊猫和北极熊基因组总丰度分别是371.8个/Mb和405.6个/Mb,二者基因组中微卫星都是单碱基重复的最多,其次是二碱基、四碱基、三碱基和五碱基,六碱基重复类型的数量最少。大熊猫和北极熊含量最丰富的重复拷贝类别主要有A、AC、AG、AAAT、AAAG、AT和C等。本研究为后续开发和筛选大量高质量的熊科物种微卫星标记提供了数据支持。In this study,the distribution of microsatellite sequences in the whole genome of giant panda well as polar bear were analyzed and compared. A total of 855 018 and 936 238 perfect microsatellite sequences with 1 ~ 6 bp nucleotide motifs were identified from giant panda and polar bear,respectively. The total length of microsatellites was 14 919 240 bp（ 0. 65% of the genome size） in giant panda,and 18 434 348 bp（ 0. 79% of the genome size） in polar bear. The abundance of microsatellites was 405. 6 no. / Mbp in polar bear,and 371. 81 no. / Mbp in giant panda. Among of which,mononucleotide repeats（ accounting for 48. 56 %） was the most abundant,followed by the dinucleotide,tetranucleotide,trinucleotide,pentanucleotide and hexanucleotide repeats. A,AC,AG,AAAT,AAAG,AT and C were the most abundant repeat units shared by the two genomes. This study can provide a basis for developing and screening a large number of highquality microsatellite markers for bear family.

关 键 词：大熊猫 北极熊 基因组 微卫星 

分 类 号：Q75[生物学—分子生物学]