含砷环境样品投加比例对体外胃肠模拟系统的砷生物可给性的影响  

Effects of dose for environmental samples containing arsenic on simulated gastrointestinal system for evaluating arsenic bioaccessibility

作　　者：杨阳[1] 张敏[1] 薛培英[1] 耿丽平[1] 刘昕然[1] 刘文菊[1] 

机构地区：[1]河北农业大学资源与环境科学学院河北省农田生态环境重点实验室,河北保定071000

出　　处：《环境与健康杂志》2014年第8期691-695,共5页Journal of Environment and Health

基　　金：河北省高等学校创新团队领军人才培育计划(LJRC016);河北省百名优秀创新人才支持计划(CPRC028)

摘　　要：目的研究质量/体积比(体积比)对不同类型和不同砷含量摄入物中砷生物可给性的影响。方法从饮品、食品和药品三类人体日常摄入物中选择砷含量不同的物质[菊花茶水、海带、牛黄解毒片(砷含量分别为0.04 mg/L和30、7.5×104mg/kg)],以不同体积比(菊花茶水/胃肠模拟液,1∶9、1∶5、1∶2),质量/体积比(干海带粉末/胃肠模拟液,1∶100、1∶50、1∶20),质量/体积比(牛黄解毒片粉末/胃肠模拟液,1∶1 800、1∶900、1∶600)投入胃肠模拟系统,消化一定时间(胃阶段1 h,肠阶段2 h)后测定胃肠液中的砷浓度,计算砷生物可给性。结果菊花茶水中砷的生物可给性随着体积比的增大而逐渐降低;在胃、肠阶段,体积比为1∶2时菊花茶水中砷的生物可给性低于体积比为1∶9时(P<0.05);且在胃阶段,体积比为1∶2时菊花茶水中砷的生物可给性低于体积比为1∶5时(P<0.05);在1∶2时砷的生物可给性最接近100%。海带中砷生物可给性随质量/体积比的增大而逐渐降低;在胃、肠阶段,质量/体积比为1∶50、1∶20时海带中砷的生物可给性均低于质量/体积比为1∶100时(P<0.05)。牛黄解毒片中砷生物可给性随着质量/体积比的增大而逐渐降低;在胃、肠阶段,质量/体积比为1∶900、1∶600时牛黄解毒片中砷的生物可给性均低于质量/体积比为1∶1 800时(P<0.05)。结论不同类型和不同砷含量摄入物中砷生物可给性均随质量/体积比(体积比)的增大而逐渐降低。评价砷含量不同的环境样品中的砷生物可给性,应根据摄入环境样品的种类和砷含量确定适宜的质量/体积比(体积比)。Objective To evaluate the effects of dose on arsenic bioaccessibility of environmental samples with different levels of arsenic. Methods Chrysanthemum tea（arsenic： 0.04 mg/L）, seaweed（arsenic： 30 mg/kg）, Niu Huang Jie Du Pian（arsenic：7.5 ×104mg/kg） were selected, a simulated gastrointestinal system established in this research was used to explore the bioaccessibility of arsenic in ingested substances by different solid-to-fluid volume ratios, including liquid-to-fluid volume ratios（tea infusions/gastrointestinal digestion） 1∶9, 1∶5, 1∶2, solid-to-fluid volume ratios（dried seaweed powder/gastrointestinal digestion） 1∶100, 1∶50, 1∶20, solid-to-fluid volume ratios（Niu Huang Jie Du Pian powder/gastrointestinal digestion） 1∶1 800, 1∶900, 1∶600. Results As for Chrysanthemum tea infusions,the arsenic bioaccessibility decreased with increasing solid-to-fluid volume ratios（liquid-to-fluid volume ratio for drinks）.In gastrointestinal stage, arsenic bioavailability at infusions-to-fluid ratio of 1∶2 to was significantly lower than that of the ratio of 1∶9. In gastric stage, arsenic bioavailability at infusions-to-fluid ratio of1∶2 was significantly lower than that of the ratio of 1 ∶5, and arsenic bioavailability at the ratio of 1 ∶2 was close to 100%. For seaweed,the bioaccessibility of arsenic decreased with increasing solid-to-fluid volume ratio, when the solid-to-fluid volume ratios ranged from 1∶50 to 1∶20, arsenic bioavailability was more stable and significantly lower than that of the ratio of 1 ∶100.For the medicine- Niu Huang Jie Du Pian,the bioaccessibility of arsenic decreased with increasing solid-to-fluid volume ratios, and the variation tendency was not conspicuous when solid-to-fluid ratio varied from 1 ∶900 to 1∶600. Conclusion The arsenic bioaccessibility of environmental samples with different levels of arsenic decreases with increasing solid-to-fluid volume ratios,so it should be done for evaluating arsenic bioaccessibility to choose

关 键 词：胃肠模拟系统 固液比 砷 生物可给性 

分 类 号：S966.4[农业科学—水产养殖]