广西巴马地区人群血清中锰超氧化物歧化酶及人类生长阻滞和DNA损伤诱导45α mRNA表达水平的研究  

Expression of MnSOD and GADD45α mRNA among populations living in Bama county of Guangxi

作　　者：覃茜[1] 张志勇[1] 于洋[1] 何敏[1] 覃健[1] 韦霄[1] 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021

出　　处：《环境与健康杂志》2014年第8期707-710,共4页Journal of Environment and Health

基　　金：国家自然科学基金(81060246;81260423);广西自然科学基金(2011GXNSFD018029);广西自然科学基金(2012GXNSFBA053109);广西科学基金(桂科青0832032;桂科自0991115);广西科学研究与技术开发项目(桂科能0815011-6-5);高等学校博士学科点专项科研基金(20104503110005)

摘　　要：目的了解广西巴马地区人群血清锰超氧化物歧化酶（Mn SOD）、人类生长阻滞和DNA损伤诱导45α（GADD45α）m RNA表达水平与家庭聚集性长寿现象的关系。方法于2011年,随机选择广西巴马地区有3~4代直系亲属的家庭成员289人,其中,长寿家族史组193人,男98人,女95人,年龄为（68.15±24.63）岁;无长寿家族史组96人,男53人,女43人,年龄为（54.09±22.83）岁。在长寿家族史组中,长寿组68人,男16人,女52人,年龄为（93.71±3.48）岁;长寿后代组125人,男82人,女43人,年龄为（54.24±19.49）岁。采用实时荧光定量RT-PCR技术检测血清Mn SOD、GADD45αm RNA的表达水平。结果各组人群血清Mn SOD m RNA的表达水平间比较,差异均无统计学意义（P〉0.05）。长寿家族史组及长寿组、长寿后代组人群血清GADD45αm RNA的表达水平均高于无长寿家族史组,且长寿家族史组女性血清GADD45αm RNA的表达水平高于无长寿家族史组,差异均有统计学意义（P〈0.05）。结论 Mn SOD、GADD45αm RNA的表达水平可能与广西巴马地区长寿家庭聚集性现象有关。Objective To investigate the expressions of Mn SOD and GADD45α m RNA in the serum among persons living in Bama area（a longevity area） in Guangxi province and explore their association with familial aggregation of longevity phenomenon.Methods A total of 289 family members were randomly selected from families in Bama, Guangxi who has 3-4 generations of immediate family members in 2011,among them,193 members in long-lived family history group（male：98,female：95） with age of（68.15±24.63） years old; 96 members in non-long-lived family history group（male： 53,female： 43） with age of（54.09±22.83） years old. In the long-lived family history group,68 members in long-lived group（male：16,female：52） with age of（93.71±3.48） years old; 125 members in offspring of long-lived group（male： 82,female： 43） with age of（54.24±19.49） years old.The real-time fluorescence quantitative RT-PCR assays was used to detect the Mn SOD,GADD45α m RNA. Results No significant differences was seen in Mn SOD m RNA expression among the groups（P 〉0.05）,while the GADD45 α m RNA expression in long-lived history family group,long-lived group and offspring of long-lived group were higher than non-longlived history family group（P〈0.05）. Conlusion Mn SOD,GADD45α m RNA expression level may be associated with longevity phenomenon in Bama county of Guangxi province.

关 键 词：血锰超氧化物歧化酶 人类生长阻滞和DNA损伤诱导45α 基因 巴马地区 

分 类 号：R181.3[医药卫生—流行病学]