右美托咪定对胎鼠离体海马神经元磷酸化CREB表达的影响  

Effects of dexmedetomidine on expression of phosphor-CREB in isolated hippocampal neurons of fetal rats

作　　者：韦祎 扈俊华[2] 梁羽冰[3] 覃怡[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021 [2]桂林医学院附属医院麻醉科 [3]广西壮族自治区肿瘤医院麻醉科

出　　处：《中华麻醉学杂志》2014年第11期1309-1311,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81373498,81060277）;广西科学研究与技术开发计划项目（1355005-4-2）;广西高校科学技术研究项目（2013ZD014）

摘　　要：目的 评价右美托咪定对胎鼠离体海马神经元磷酸化环磷酸腺苷反应元件结合蛋白（p-CREB）表达的影响.方法 孕16 ～ 18 d SD大鼠,拉颈处死,剖腹取胎鼠,分离海马神经元.将神经元接种于培养液中,培养至第8天,采用随机数字表法分为4组（n=12）：对照组（C组）、右美托咪定0.001 μmol/L组（D1组）、右美托咪定0.010μmol/L组（D2组）和右美托咪定0.100 μmol/L组（D3组）.C组不做任何处理,D1-3组培养液中加入右美托咪定,终浓度分别为0.001、0.010和0.100 μmol/L,孵育3.5h.采用流式细胞仪检测海马神经元凋亡情况,采用Western blot法检测海马神经元p-CREB表达.结果 与C组比较,D1-3组海马神经元的凋亡率降低,p-CREB表达上调（P＜0.05）.结论 右美托咪定通过上调p-CREB表达抑制胎鼠离体海马神经元凋亡.Objective To evaluate the effects of dexmedetomidine on the expression of phosphor-cAMP response element binding protein （p-CREB） in isoloated hippocampal neurons of fetal rats.Methods SpragueDawley rats on 16-18 days of gestation were sacrificed and the fetal rats were obtained.The hippocampi of fetal rats were isolated and hippocampal neurons were seeded in culture medium for 8 days.The cells were then divided into 4 groups （n =12 each） using a random number table：control group （group C）,dexmedetomidine 0.001 μmol/L group （group D1）,dexmedetomidine 0.010 μmol/L group （group D2）,and dexmedetomidine 0.100μmol/L group （group D3）.In D1.3 groups,dexmedetomidine with the final concentrations of 0.001 μmol/L,0.010 μmol/L,and 0.100 μmol/L was added to the culture medium,respectively,and then the cells were incubated for 3.5 h.The apoptosis in hippocampal neurons was detected by flow cytometry.The expression of p-CREB in hippocampal neurons was determined by RT-PCR and Western blot.Results Compared with group C,apoptosis rate was significantly decreased and the expression of p-CREB was up-regulated in D1.3 groups.Conclusion Dexmedetomidine inhibits apoptosis in isolated hippocampal neurons of fetal rats by up-regulating the expression of p-CREB.

关 键 词：右美托咪啶 CAMP反应元件结合蛋白质 胎儿 海马 神经元 

分 类 号：R614[医药卫生—麻醉学]